Laboratory Study
Effect of in vitro manipulation on mitochondrial membrane potential of human oocytes
Bi Xingyu, Zhang Dongdong, Zhu Pengfei, Su Dan, Wu Xueqing
Published 2017-05-25
Cite as Chin J Reprod Contracept, 2017,37(05): 389-391. DOI: 10.3760/cma.j.issn.2096-2916.2017.05.009
Abstract
ObjectiveTo compare the effects of different operations on the mitochondrial membrane potential of oocytes in vitro.
MethodsThe mitochondrial membrane potential of oocytes, treated by oocyte maturation in vitro, vitrification and intracytoplasmic sperm injection (ICSI), were detecdted by JC-1 kit.
ResultsNo significant changes were observed in the mitochondrial membrane potential during the oocytes maturation in vitro, vitrification of oocytes and the micromanipulation of oocytes. At the same time, there was a significant decrease in the mitochondrial membrane potential of oocytes after the failure of in vitro maturation, and the failure of fertilization after single sperm injection.
ConclusionIn vitro routine operation has no obvious effect on the mitochondrial membrane potential of oocyte, but there is a close relationship between mitochondrial membrane potential and oocyte quality. Therefore, mitochondrial membrane potential can be used as a new indicator to evaluate the quality and a supplement to the morphological evaluation.
Key words:
Oocyte; Mitochondrial membrane potential; Apoptosis
Contributor Information
Bi Xingyu
Reproductive Medical Center, Maternal and Children Health Hospital of Shanxi Province, Taiyuan 030013, China
Zhang Dongdong
Reproductive Medical Center, Maternal and Children Health Hospital of Shanxi Province, Taiyuan 030013, China
Zhu Pengfei
Reproductive Medical Center, Maternal and Children Health Hospital of Shanxi Province, Taiyuan 030013, China
Su Dan
Reproductive Medical Center, Maternal and Children Health Hospital of Shanxi Province, Taiyuan 030013, China
Wu Xueqing
Reproductive Medical Center, Maternal and Children Health Hospital of Shanxi Province, Taiyuan 030013, China
