A novel micro-straw (LSL straw): the reliable cryopreservation vehicle for rare or very low concentrations of human sperm (83 cases reported)
Yunjing Xue, Yuan Xu, Feng Liu, Zijue Zhu, Yuehua Gong, Xiaobo Wang, Jianlin Hu, Jian Sun, Wei Chen, Zhenbo Zhang, Yu Wu, Zheng Li
Abstract
ObjectiveTo analyze the pregnancy outcome of cases who underwent intracytoplasmic sperm injection (ICSI) with frozen-thawed sperm, which was rare and weak, cryopreserved by a novel micro-straw (LSL micro-straw).
MethodsThe present study retrospectively analyzed the post-thaw results of cryopreserved human sperm from 83 severe infertility male patients (including oligospermia and asthenospermia). Then we investigated the evolution of embryo development and pregnancy outcome in 23 patients underwent ICSI.
ResultsAbout 89.5% (94/105) frozen-thawed samples could observe motile sperm in quality control. About 73.9% (17/23) sperm were available in 18 ICSI cycles. The fertilization rate, the cleavage rate, the available embryo rate and the high-quality embryo rate were 78.0%, 92.5%, 62.7% and 78.0%, respectively. The implantation rate, the clinical pregnancy rate and the cumulative live birth rate were 31.1%, 52.5% and 55.6%, respectively. Totally 12 live births were obtained with no birth defects reported.
ConclusionLSL micro-straw is a safe and feasible new carrier for cryopreservation of oligospermia and asthenospermia, which can be widely applicated in assisted reproductive technology.
Key words:
Rare human spermatozoa; Freezing and thawing; Novel micro-straw (LSL micro-straw); Intracytoplasmic sperm injection (ICSI); Pregnancy outcomes
Contributor Information
Yunjing Xue
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Yuan Xu
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Feng Liu
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Zijue Zhu
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Yuehua Gong
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Xiaobo Wang
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Jianlin Hu
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Jian Sun
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Wei Chen
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Zhenbo Zhang
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Yu Wu
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
Zheng Li
Department of Andrology, Center for Men’s Health, Department of ART, Institute of Urology, Urologic Medical Center, Shanghai General Hospital, Shanghai Key Lab of Reproductive Medicine, Shanghai Jiao Tong University, Shanghai 200080, China
