Clinical Study
Effect of vitrification on the expression of imprinted gene Snrpn of neonatal placental tissues
Jianfeng Yao, Yanfang Huang, Rongfu Huang, Suxia Lin, Caiqiong Guo, Peiya Wu, Youzhu Li, Jifeng Hu
Published 2019-02-25
Cite as Chin J Reprod Contracep, 2019, 39(2): 97-102. DOI: 10.3760/cma.j.issn.2096-2916.2019.02.003
Abstract
ObjectiveTo investigate the effect of vitrification on the expression of imprinted geneSnrpn of neonatal placental tissues.
MethodsThe neonatal placental tissue after delivery from infertility patients undergoingin vitro fertilization-embryo transfer (IVF-ET) treatment at the Reproductive Medicine Center of Quanzhou Maternal and Child Health Hospital during the period from 1 March 2015 to 31 October 2017, were collected respectively. Samples were divided into fresh group and vitrified group according to the pregnancy outcomes following fresh or vitrified embryo transfer. The placenta tissue from the natural pregnancy was collected as control group. Real-time RT-PCR and Western blotting were used to detect theSnrpn mRNA levels and SNRPN protein levels in placenta tissues from the above three groups.
ResultsCompared with control group, the expression ofSnrpn mRNA and SNRPN protein in the placenta from the fresh group and the vitrified group increased, with a statistically significant difference (mRNA: allP<0.001; protein:P=0.008,P=0.005). There was no significant difference between the fresh group and the vitrified group (P=0.212,P=0.286).
ConclusionIVF may affect the imprinting geneSnrpn transcriptional expression in mRNA and protein levels to some extent, and the vitrification technology may not increase or reduce this impact.
Key words:
Fertilizationin vitro; Embryo transfer; Placenta; Snrpn gene
Contributor Information
Jianfeng Yao
Reproductive Medicine Center, Quanzhou Maternity &
Child Healthcare Hospital, Quanzhou 362000, China
Yanfang Huang
Health Checkup Center, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, China
Rongfu Huang
Clinical Testing Center, The Second Affiliated Hospital, Fujian Medical University, Quanzhou 362000, China
Suxia Lin
Reproductive Medicine Center, Quanzhou Maternity &
Child Healthcare Hospital, Quanzhou 362000, China
Caiqiong Guo
Reproductive Medicine Center, Quanzhou Maternity &
Child Healthcare Hospital, Quanzhou 362000, China
Peiya Wu
Reproductive Medicine Center, Quanzhou Maternity &
Child Healthcare Hospital, Quanzhou 362000, China
Youzhu Li
Reproductive Medicine Center, The First Affiliated Hospital, Xiamen University, Xiamen 361002, China
Jifeng Hu
Health Checkup Center, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350004, China