Clinical Study
Application of recombinant human growth hormone in patients with previous in vitro fertilization failure
Lifeng Tian, Min Gao, Qiong Su, Dingfei Xu, You Li, Qiongfang Wu
Published 2019-09-25
Cite as Chin J Reprod Contracep, 2019, 39(9): 730-734. DOI: 10.3760/cma.j.issn.2096-2916.2019.09.008
Abstract
ObjectiveTo investigate the effect of recombinant human growth hormone (rhGH) on embryo quality and clinical outcome of patients used an early follicular phase prolonged protocol with previous in vitro fertilization (IVF) failure.
MethodsFrom April 2017 to April 2018, 54 patients who had pregnancy failure due to no available embryo or poor quality of embryo in the previous cycle with early follicular phase prolonged protocol were selected for the study. Patients used an early follicular phase prolonged protocol both before and after in the two cycles. In the second cycle, rhGH pretreatment was added for 4 weeks (GH group), and rhGH was maintained until human chorionic gonadotropin (hCG) injection day during ovulation induction. The improvement of embryo quality and the pregnancy outcome after rhGH addition was observed by self-control method.
ResultsThere were no significant differences in the duration of gonadotropin (Gn) used, total dosage of Gn used, the endometrial thickness and estrogen levels on hCG injection day, the number of oocytes retrieved, two pronucleus (2PN) cleavage rate, embryo transfer number per cycle between the two groups (P>0.05). In GH group, the progesterone levels on hCG injection day [(0.55±0.29) μg/L], the early abortion rate (7.69%) and the cancellation cycle rate due to poor embryo quality (0%) were significantly lower than those in control group [(0.80±0.31) μg/L, P<0.001; 66.67%, P=0.042; 24.07%, P<0.001]. The 2PN fertilization rate (64.36%), the available embryo rate (56.08%), the clinical pregnancy rate (50.98%) and the implantation rate (42.39%) in GH group were significantly higher than those in control group (53.90%, 31.75%, 7.50%, 4.84%) (all P<0.001).
ConclusionLow dose rhGH pretreatment can significantly improve embryo quality and pregnancy outcome in patients who had previous pregnancy failure due to poor embryo quality, and received an early follicular phase prolonged protocol in IVF cycles.
Key words:
Growth hormone; Clinical pregnancy rate; Fertilization in vitro; Embryo transfer; Embryo quality; Early follicular phase prolonged protocol
Contributor Information
Lifeng Tian
Department of Human Assisted Reproduction Center, Women and Children Health Care Hospital of Jiangxi Province, Nanchang 330006, China
Min Gao
Qiong Su
Dingfei Xu
You Li
Qiongfang Wu
