To investigate the factors of repeated transplant failure, and the correlation between repeated transplantation failure with general characteristics, embryonic laboratory conditions and metabolic indicators etc.

From March 2018 to December 2018, 3263 female patients who received routine in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment and nutrition consultation in Shanxi Maternal and Child Health Care Hospital were selected to analyze the related factors which affected the failure of repeated transplantation. The patients were divided into successful transplantation group (control group, 620 cases) and repeated transplantation failure group (failure group, 368 cases) depend on the result of transplantation. Those patients were observed and compared between the two groups about those factors.

1) Basic indicators: the age of control group was younger than that of the failure group (P=0.000 1). The duration of infertility in control group was shorter than that in the failure group (P=0.021). The rate of secondary infertility, the rate of unbalanced dietary, the rate of ICSI pregnancy in control group were lower than those in the failure group (all P=0.000 1). 2) Laboratory indicators: the number of eggs obtained, the number of M_II eggs, the number of two pronuclear (2PN), the number of cleavage eggs, the number of embryos, the number of remaining frozen embryos in control group were more than those in the failure group (all P=0.000 1). 3) Metabolic index: the ratio of cholesterol (TC) ≥5.2 mmol/L, the ratio of triglycerides (TG)≥1.7 mmol/L, the ratio of high density lipoprotein (HDL)<1.29 mmol/L, the ratio of albumin (<40 g/L) in control group were lower than those in the failure group (P=0.044, P=0.027, P<0.001, P<0.001). 4) Logistic regression analysis: infertility duration, 2PN number, number of embryos prepared, HDL<1.29 mmol/L and albumin<40 g/L were closely related to repeated transplantation failure.

Dietary imbalance leads lower albumin and HDL, which increases the occurrence of metabolic syndrome, impairs the number of 2PN and the number of embryos, and finally affects the blood circulation, leading to the failure of repeated transplantation.

Albumin; Lipid; Metabolic syndrome; Dietary balance; Repeated transplantation failure