Experimental Article
Differential expression of long no-coding RNA in human intracranial arteriovenous malformation tissue
Xiong Li, Jun Wu, Fuxin Lin, Suo Wang
Published 2016-07-28
Cite as Chin J Neurosurg, 2016, 32(7): 721-726. DOI: 10.3760/cma.j.issn.1001-2346.2016.07.017
Abstract
ObjectiveTo investigate the differential expression of long no-coding RNA(LncRNA)in human brain arteriovenous malformation(AVM)tissue using an Arraystar-LncRNA chip.
MethodsThe vascular nidi of 14 patients with AVM used as the specimens of the test group and operated in Beijing Tiantan Hospital, Capital Medical University were selected. The scalp blood vessel or superficial temporal artery segment of the same patient was used as a control specimen. Arraystar-LncRNA chip was used to conduct the expression profile analysis of LncRNA and mRNA. The maximum expression difference of 8 LncRNAs was selected for PCR validation.
ResultsAfter screening and validation, in the 2 8012 detectable LncRNAs, 1 931 were up-regulated for ≥2 times and 450 were up-regulated for ≥4 times compared with the control group; 1 852 were down-regulated for ≥2 times and 719 were down-regulated for ≥4 times. In the 21 780 detectable mRNAs, 1 577 were up-regulated for ≥2 times, 450 were up-regulated for ≥4 times; and 1 699 were down-regulated for ≥2 times and 681 were down-regulated for ≥4 times. The bioinformatics and pathway analyses for mRNAs showed that the differential expression of 8-code mRNAs(FDXR, SYNE2, FCGR3A, NDUFS4, COX5A, OPA1, ETFA, and LPL)were most obvious in comparison of the test group and the control group. These mRNAs were associated with mitochondrial electron respiratory chain function, interaction between cytokines, stress response, lipoprotein lipase, and cytoskeleton associated protein. PCR validation for mRNAs associated with these LncRNAs showed that there were significant differences in the differential expression of 4 LncRNAs(codes ENST00000423394(P=0.03), ENST00000444114(P=0.01), TCONS_00013855(P=0.01), and ENST00000452148(P=0.01).
ConclusionThe occurrence and development mechanism of intracranial AVM may be associated with the mitochondrial NADPH oxidoreductase, lipoprotein lipase, and optic atrophy associated protein-related down-regulation of LncRNA expression.
Key words:
Intracranial arteriovenous malformations; Gene expression profile; Biological processes; Long no-coding RNA
Contributor Information
Xiong Li
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
Jun Wu
Fuxin Lin
Suo Wang
