Experimental Article
Experimental research of the effect of TUG1 gene silencing on the biological function of brain glioma
Bin Wang, Weitao Man, Yongkai Chang, Junhai Hao, Jun Yang
Published 2016-09-28
Cite as Chin J Neurosurg, 2016, 32(9): 940-945. DOI: 10.3760/cma.j.issn.1001-2346.2016.09.020
Abstract
ObjectiveTo observe the effect of TUG1 gene silencing on glioma cell growth and apoptosis.
MethodsA slow virus vector pLenti6.3-EGFP of carrying shRNA-TUG1 was constructed and then transfected into human glioma U251 cells. The expression levels of TUG1 LncRNA gene in U251 cells were detected by using the real-time fluorescence quantitative PCR. The inhibitory effect of the interference vector on LncRNA TUG1 gene was observed. Flow cytometry and CCK-8 assay were used to observe the effect of interfering TUG1 gene on U251 cell proliferation and apoptosis.
ResultsThe results of real-time fluorescent quantitative PCR detection showed that as for the relative amount of LncRNA TUG1, there were significant differences among the U251 blank control group (0.40±0.05), the negative control group (1.01±0.12), siRNA-1 (0.09±0.01), siRNA-2 (0.32±0.01), siRNA-3 (0.18±0.04), and siRNA-4 (0.18±0.02) (P<0.05). The siRNA-1 with the best interference effect was selected for subsequent interference experiments. The apoptotic rate of the TUG1 interference group (11.58±0.11%) was higher than that of the blank control group (8.55±0.23%) and the negative control group (9.14±0.17%) (all P<0.05); the cell proliferation capacity of the TUG1 interference group (0.64±0.23) was lower than that of the blank control group (1.07±0.50) and the negative control group (0.94±0.42) (all P<0.05).
ConclusionTUG1 gene silencing may inhibit the proliferation of U251 glioma cells and promote cell apoptosis.
Key words:
Glioma; Gene silencing; Real-time polymerase chain reaction; Apoptosis; Taurine up-regulated gene 1
Contributor Information
Bin Wang
Department of Neurosurgery, Beijing Fuxing Hospital, Capital Medical University, Beijing 100038, China
Weitao Man
Yongkai Chang
Junhai Hao
Jun Yang