Effect of Berberine on endoplasmic reticulum stress and insulin resistance in skeletal muscle of metabolic syndrome rats and its mechanism
Hong Zhang, Tao Fang, Fengshi Tian, Yanbo Di, Na Shen, Ying Chen, Qiang Chen, Feng He, Yang Bai, Huanming Li
Published 2019-08-14
Cite as Chin J Geriatr, 2019, 38(8): 924-928. DOI: 10.3760/cma.j.issn.0254-9026.2019.08.024
Abstract
ObjectiveTo investigate the effect of Berberine on insulin resistance and its mechanism in Otsuka Long-Evans Tokushima Fatty(OLETF)rats with metabolic syndrome(MS).
MethodsLETO(Long-evans Tokushima Otsuka)rats(the control group receiving standard normal diet, n=10)and diabetic OLETF rats(the MS group receiving high-fat diet for 24 weeks, n=30). Rats in the MS group were randomly divided into 3 subgroups(n=10, each subgroup). Each subgroup was gavaged with normal saline, high-dose Berberine(100 mg·kg-1·d-1)and low-dose Berberine(50 mg·kg-1·d-1)respectively, and the high-fat diet remained unchanged.After 6 weeks of berberine treatment, body weight, blood glucose and lipid metabolism parameters were determined.The oral glucose tolerance test(OGTT)and insulin tolerance test(ITT)were used to detect insulin resistance.Expression levels of the protein and mRNA of 78 kDa glucose-regulated protein(GRP78), Caspase-12 and CCAAT/enhancer-binding protein(C/EBP)homologous protein(CHOP)in skeletal muscles were detected by Western blot and RT-PCR.
ResultsAfter Berberine treatment, the body weight, fasting plasma glucose, fasting insulin[(28.9±2.0) mU/L, (31.5±2.4) mU/L vs.(36.9±4.7) mU/L], total cholesterol, triglycerides, and low-density lipoprotein cholesterol were decreased, while the high-density lipoprotein cholesterol(HDL-C)levels were increased in MS rats with high-dose berberine and low-dose berberine as compared with the control group(P<0.05)respectively.Berberine treatment could reduce the protein and mRNA expression levels of GRP78, Capase-12 and CHOP in the skeletal muscle of MS rats(P<0.05).
ConclusionsBerberine may alleviate insulin resistance in rats with metabolic syndrome by reducing endoplasmic reticulum stress in skeletal muscle.
Key words:
Metabolic syndrome X; Berberine; Muscle, skeletal; Insulin antibodies
Contributor Information
Hong Zhang
Department of Cardiology, Tianjin Chest Hospital, Tianjin 300222, China
Tao Fang
Department of Metabolic Syndrome Laboratory, the 4th Center Hospital of Naikai University, Tianjin 4th Center Hospital, Tianjin 300140, China
Fengshi Tian
Department of Metabolic Syndrome Laboratory, the 4th Center Hospital of Naikai University, Tianjin 4th Center Hospital, Tianjin 300140, China
Yanbo Di
Department of Metabolic Syndrome Laboratory, the 4th Center Hospital of Naikai University, Tianjin 4th Center Hospital, Tianjin 300140, China
Na Shen
Department of Metabolic Syndrome Laboratory, the 4th Center Hospital of Naikai University, Tianjin 4th Center Hospital, Tianjin 300140, China
Ying Chen
Department of General Surgery, the 4th Center Hospital of Nankai University, Tianjin 4th Center Hospital, Tianjin 300140, China
Qiang Chen
Department of Chinese Medicine, the 4th Center Hospital of Nankai University, Tianjin 4th Center Hospital, Tianjin 300140, China
Feng He
Department of Cardiology, the 4th Center Hospital of Nankai University, Tianjin 4th Center Hospital, Tianjin 300140, China
Yang Bai
Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China
Huanming Li
Department of Cardiology, the 4th Center Hospital of Nankai University, Tianjin 4th Center Hospital, Tianjin 300140, China
