Survivin as a factor of radioresistance to high-LET carbon ions in human hepatoma SMMC.7721 cells
JIN Xiao-dong, GONG Li, LI Qiang, HAO Ji-fang, LI Ping, WU Qing-feng, HE Ling, LIU Xin-guo, DAI Zhong-ying
Published 2009-01-25
Cite as Chin J Radiol Med Prot, 2009,29(01): 1-4. DOI: 10.3760/cma.j.issn.0254-5098.2009.01.001
Abstract
Objective To investigate the influences of survivin down-regulation on cell G2/M phase arrest,apeptosis and sensitivity to carbon ion irradiation. Methods Small interfering RNA (siRNA) targeting survivin mRNA was designed, in vitro chemo-synthesized and transfected into SMMC-7721 cells. Survivin mRNA expression in SMMC-7721 cells was measured by real-time PCR, and the apeptotic rates by Annexin-FTTC at 24 and 48 h after transfection. Cell G2/M phase arrest after transfection was assessed with flow eytometry as well. Cellular sensitivity to high-LET carbon ions was determined by means of colony-forming assay. Results The expressions of survivin at mRNA level were down-regulated to be 59% and 39% in relation to the non-treated cells at 24 and 48 h after siRNA transfeetion, respectively. G2/M phase arrest in SMMC-7721 cells at 24 h after transfection was observed while much more obvious at 48 h. The apeptotic rate of SMMC-7721 cells was 21.41 % at48 h after survivin siRNA transfection, which was significantly higher than that of the cells transfected with negative siRNA. Moreover, a decreased clonogenic survival in siRNA treated group was shown. Conclusion Down-regulation of survivin gene expression in SMMC-7721 cells by siRNA could effectively induce cell apeptosis and G2/M phase arrest, and enhance the cellular radiosensitivity to high-LET heavy ions.
Key words:
SMMC-7721 cell; Survivin; Apoptosis; RNA interference
Contributor Information
JIN Xiao-dong
Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
GONG Li
Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
LI Qiang
Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
HAO Ji-fang
Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
LI Ping
Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
WU Qing-feng
Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
HE Ling
Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
LIU Xin-guo
Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China
DAI Zhong-ying
Institute of Modern Physics, Chinese Academy of Science, Lanzhou 730000, China