Radiological Biology
Effect of reduced glutathione tablets on radiation-induced lung injury in mice
Shugen Qu, Fan Chen, Yuxia Zhang, Bin Li, Zhaofen Wang, Cai Wang, Ruixing Feng, Kunling Wang
Published 2016-02-25
Cite as Chin J Radiol Med Prot, 2016, 36(2): 105-110. DOI: 10.3760/cma.j.issn.0254-5098.2016.02.005
Abstract
ObjectiveTo study the effects and underlying mechanism of reduced glutathione (GSH) tablets on radiation-induced lung injury in mice.
MethodsA total of 100 BALB/c mice were randomly divided into 5 groups: 15 Gy, 15 Gy+ GSH, 30 Gy, 30 Gy+ GSH, and healthy control group. Mice of GSH groups were intraperitoneally injected with 0.2 ml of 240 mg/ml GSH, and other two radiation alone groups were treated with an equal volume of saline. The mice were delivered with 15 Gy (30 Gy) of X-rays to build a RILI model. At 0, 1, 2, 3 weeks after irradiation, blood samples were collected for ELISA measurements of MMP-9, TIMP-1, IL-4 and IL-6, the lung pathology was detected by HE, and the mouse weight and immune system were also analyzed.
ResultsThe mice spleen index fell sharply after irradiation and the decline rate decreased with increasing dose (F=29.84, P<0.05), then this index slowly increased at 1 week after irradiation along with radiation dose (F=13.91, 4.61, P<0.05). The expressions of MMP-9 and IL-6 in blood serum increased along with radiation dose (F=81.27, 10.86, P<0.05), but those of TIMP-1 and IL-4 decreased. In addition, following irradiation, the expression of MMP-9 increased at first and then decreased (F=52.22, P<0.05), while the expressions of TIMP-1 and IL-4 dropped immediately at first, and then increased until at 3 week after radiation(F=138.96, 8.48, P<0.05), and the expression of IL-6 gradually increased.
ConclusionsGSH has significant radioresistant effect on RILI, especially at low dose.
Key words:
Mice; Glutathione; Radiation-induced lung injury; Cytokines
Contributor Information
Shugen Qu
Department of Radiation Oncology, Qinghai University Affiliated Hospital, Xining 810001, China
Fan Chen
Department of Radiation Oncology, Qinghai University Affiliated Hospital, Xining 810001, China
Yuxia Zhang
Department of Public Health, Qinghai University Medical College, Xining 810001, China
Bin Li
Department of Public Health, Qinghai University Medical College, Xining 810001, China
Zhaofen Wang
Department of Public Health, Qinghai University Medical College, Xining 810001, China
Cai Wang
Department of Radiation Oncology, Qinghai University Affiliated Hospital, Xining 810001, China
Ruixing Feng
Department of Radiation Oncology, Qinghai University Affiliated Hospital, Xining 810001, China
Kunling Wang
Department of Radiation Oncology, Qinghai University Affiliated Hospital, Xining 810001, China
