Radiological Biology
Protective effects of ferulic acid on mice bone marrow hematopoietic damage induced by irradiation
Shuai Shao, Mei Tian, Jianxiang Liu, Qiao Gou, Xuesong Qi, Chunyan Wang, Gonglin Qu, Chen Li, Xu Su
Published 2017-08-25
Cite as Chin J Radiol Med Prot, 2017, 37(8): 570-575. DOI: 10.3760/cma.j.issn.0254-5098.2017.08.002
Abstract
ObjectiveTo observe the effect and the mechanisms of ferulic acid on radiation-induced damage of mice peripheral blood and bone marrow hematopoietic function.
MethodsNinety-six mice were randomly divided into sham irradiation group, irradiation group, positive drug group and 10, 30, 90 mg·kg-1·d-1 ferulic acid group, 16 mice per group. Mice were exposed to 3.5 Gy γ-rays 24 h after first drug taken. Then, mice were given drugs for 7 d after irradiation. White blood cells in peripheral blood of 10 mice per group were counted 2 d before irradiation and 3, 7, 10, 15 and 22 days after irradiation. The bone marrow of the other six mice was taken to detect the micronuclei frequency of polychromatic erythrocyte, the hematopoietic progenitor cell colony formation capacity, Thbd and HMGB1 protein expressions in mice bone marrow on the seventh day after irradiation.
ResultsCompared with the irradiation alone group, the treatment of mice with ferulic acid 90 mg·kg-1·d-1 increased the number of white blood cells in peripheral blood at 3, 10, 15 and 22 d after irradiation (t=2.267, 2.399, 1.945, 2.828, P<0.05). Treatment with mice with ferulic acid 90 mg·kg-1·d-1 decreased the micronuclei rate of erythrocytes in irradiated bone marrow (t=4.013, P<0.05), increased the clone numbers of CFU-E, BFU-E and CFU-GM of hematopoietic progenitor cells (t=2.366, 2.953, 3.115, P<0.05), improved the relative expression of the Thbd protein in bone marrow and the HMGB1 protein in nuclear (t=17.75, 23.39, P<0.01).
ConclusionsFerulilc acid could protect the bone marrow hematopoietic of mice exposed to irradiation by regulating the expressions of Thbd and HMGB1 protein, and then accelerate the peripheral cells recovery.
Key words:
Ferulic acid; Radiation protection; Bone marrow hematopoiesis; Thrombomodulin; High mobility group box-1 protein
Contributor Information
Shuai Shao
Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China
Mei Tian
Jianxiang Liu
Qiao Gou
Xuesong Qi
Chunyan Wang
Gonglin Qu
Chen Li
Xu Su