B lymphocyte counts and Toil-like receptor 9 mRNA expression on cell from HIV-1 infected patients
SUN Hong, GENG Wen-qing, CUI Hua-lu, ZHAO Min, HAN Xiao-xu, ZHANG Zi-ning, LIU Jing, DAI Di, WANG Ya-nan, JIANG Yong-jun, SHANG Hong
Published 2010-09-30
Cite as Chin J Microbiol Immunol, 2010,30(09): 860-863. DOI: 10.3760/cma.j.issn.0254-5101.2010.09.017
Abstract
Objective To study the B lymphocytes counts and the expression of TLR9 mRNA on B lymphocytes in peripheral blood from Chinese HIV-infected patients.Methods The cells from peripheral blood were stained with antibodies labeled with fluorescence and B lymphocytes were counted with flow cytometry.Using the method of magnetic activated cell sorting and real-time PCR,the expression of TLR9 mRNA was measured.Results The B lymphocytes counts in HIV/AIDS patients was significantly lower than that of healthy controls(P ＜0.01).The B lymphocytes counts in HIV/AIDS patients positively correlated with the CD4 +T cells counts(r =0.534,P = 0.006).The expression of TLR9 mRNA on B lymphocytes in HIV/AIDS patients was significantly lower than that of healthy controls(P =0.023),and positively correlated with the CD4 + T cells counts(r = 0.390,P = 0.040).Conclusion The B lymphocytes counts and the expression of TLR9 mRNA on B lymphocytes in Chinese HIV/AIDS patients were decreased due to HIV infection,which may correlate with disease progression.
Key words:
HIV; AIDS; B lymphocyte; Toll-like receptor
Contributor Information
SUN Hong
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
GENG Wen-qing
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
CUI Hua-lu
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
ZHAO Min
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
HAN Xiao-xu
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
ZHANG Zi-ning
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
LIU Jing
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
DAI Di
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
WANG Ya-nan
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
JIANG Yong-jun
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
SHANG Hong
Key Laboratory of AIDS Immunology of Ministry of Health,the First Hospital of China Medical University,Shenyang 110001,China
