Objective To detect the difference of cytokines and antibodies productions by immunologic system from mice vaccinated with recombinant P6 protein with different immunization routes and adiuvants.Methods 6 weeks female BALB/c mice were vaccinated with meombinant protein P6 combined with A1(OH)3,CpG ODN or the mix of them through intramuscular and intranasal.The mice were boosted twice with the same dose by the same route.Serum and respiratory tract specimen were collected to detect rP6 specific antibodies by ELISA.Results In the 6th week after immunization.The higher titer of serum rP6 specific IgG antibody were detected from the two groups vaccinated with A1(OH)3+CpG ODN+rP6 by intramuscular and vaccinated with CpG ODN+rP6 by intranasal(F=41.259.P=0.000).The ELISPOT experiment showed that,Inoculation with the CpG ODN+rP6 either by intranasal or intramuscular immunization could induce a large number of antigen-specific T lymphocyte activation.In addition.The mice vaccinated with the CpG ODN+rP6 through intranasal can be detected high titer rP6 specific mucosal IgA antibody(F=70.966.P=0.000).Conclusion Inoculation with the CoG ODN+rP6 by jntranasal immunization not only can induce high-titer serum IgG antibody,but also can induce high-titer mucosal IsA antibody and CD4+Th1.CD8+T lymphoeyte activation.