Antimicrobial resistance patterning and pulsed field gel electrophoresis (PFGE) typing for non -typhoidal Salmonella isolated from diarrhea cases in Guangdong province, China
LI Bai-sheng, KE Bi-xia, HE Dong-mei, TAN Hai-ling, WANG Chen, LIANG Zhao-ming, LIU Mei-zhen, CHEN Jing-diao, KE Chang-wen
Published 2012-06-30
Cite as Chin J Microbiol Immunol, 2012,32(06): 542-548. DOI: 10.3760/cma.j.issn.0254-5101.2012.06.012
Abstract
Objective To investigate the antimicrobial resistance pattern of non-typhoidal Salmonella isolated from diarrhea cases in Guangdong province,China.The multidrug-resistant strains were analyzed by pulsed field gel electrophoresis(PFGE) typing.Methods All the non-typhoidal Salmonella strains isolated between 2009 and 2011 were serotyped,then the antimicrobial resistance was detected by the disk diffusion method and molecular typed by PFGE.Results 91.76% (256/279) S.typhimurium isolates were multiple resistant to 3 and more antimicrobials.Forty S.typhimurium isolates were multiple resistant to 9 and more antimicrobials and 3 out of which were multiple resistant to all the 12 antimicrobials in vitro.96.91% (94/97) Salmonella I4,5,12:i:-isolates were multiple resistant to 3 and more antimicrobials.Nine Salmonella I4,5,12:i:- isolates were multiple resistant to 9 and more antimicrobials and I out of which was multiple resistant to all the 12 antimicrobials1 in vitro.47% (47/100) S.enteritidis isolates were multiple resistant to 3 and more antimicrobials.Only 1 S.enteritidis isolates was multiple resistant to 9 and more antimicrobials.4.27% (27/632) non-typhoidal Salmonella isolates was resistant to ciprofloxacin,including 17 S.typhimurium and 6 Salmonella 14,5,12:i:- isolates.Also,there were 3 1.96% ( 202/632 ) non-typhoidal Salmonella isolates was intermediary to ciprofloxacin.The PFGE patterns of the predominant strains which were highly resistant and multidrug-resistant had different genotypes and demonstrated significant genetic diversity.Conclusion The situation about the multiple antimicrobial resistances of non-typhoidal Salmonella in Guangdong province has showed the prevalent problem.The PFGE types of the multiple drug-resistant strains prompted these strains were come from different clones.This requires that we continue to strengthen the resistance monitoring and control of the rational use of antibiotics.
Key words:
Non-typhoidal Salmonella; Antimicrobial resistance pattern; Pulsed field gel electrophoresis
Contributor Information
LI Bai-sheng
Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510300,China
KE Bi-xia
Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510300,China
HE Dong-mei
Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510300,China
TAN Hai-ling
Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510300,China
WANG Chen
Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510300,China
LIANG Zhao-ming
Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510300,China
LIU Mei-zhen
Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510300,China
CHEN Jing-diao
Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510300,China
KE Chang-wen
Guangdong Provincial Center for Disease Control and Prevention,Guangzhou 510300,China