Basic Immunology
Effects of original antigenic sin on the immune response to inactivated influenza A virus subtype H5N1 vaccine
Zhenyuan Xie, Na Peng, Yanan Zhang, Shubao Mao, Ze Chen, Haiyan Chang
Published 2016-08-31
Cite as Chin J Microbiol Immunol, 2016, 36(8): 566-571. DOI: 10.3760/cma.j.issn.0254-5101.2016.08.002
Abstract
ObjectiveTo investigate the effects of original antigenic sin caused by previous exposure to influenza A virus subtype H1N1 on the immune response to inactivated H5N1 vaccine.
MethodsIn this study, the BALB/c mice were first infected with A/PR8 (H1N1) virus or immunized with inactivated vaccine to induce immune responses against the A/PR8 virus. Then they were injected once with inactivated H5N1 vaccine at dosages of 0.01 μg, 0.1 μg and 1 μg, respectively. The levels of IgG and neutralizing antibodies in serum samples were detected after immunization. Four weeks after immunization, the mice were challenged with a lethal dose of H5N1 virus. Some indicators including the survival rate, body weight loss and residue virus titer in lung were recorded for further evaluation.
ResultsThe pre-existing anti-A/PR8 antibodies in mice didn′t alleviate the immune responses to inactivated H5N1 vaccine.
ConclusionThis study indicates that the original antigenic sin associated with the previous exposure to A/PR8 virus has no significant effect on the immune efficacy of H5N1 vaccine.
Key words:
Influenza vaccine; Original antigenic sin; Influenza A virus subtype H5N1
Contributor Information
Zhenyuan Xie
Shanghai Xuhui District Xietu Street Community Health Center, Shanghai 200032, China
Na Peng
School of Life Sciences, Hunan Normal University, Changsha 410081, China
Yanan Zhang
School of Life Sciences, Hunan Normal University, Changsha 410081, China
Shubao Mao
Shanghai Institute of Biological Products Co., Ltd., Shanghai 200052, China
Ze Chen
Shanghai Institute of Biological Products Co., Ltd., Shanghai 200052, China
Haiyan Chang
School of Life Sciences, Hunan Normal University, Changsha 410081, China
