Basic Immunology
Analysis of TCR CDR3 sequences of E7/HLA-DR tetramers-bound CD4+ T cells from tuberculosis patients by using tetramers constructed with different HLA-DRB1 alleles
Yichuan Gan, Cong Wang, Yimin Fang, Yanan Yao, Xiaoxin Tu, Yanming Shen, Xiaomin Lai
Published 2017-02-28
Cite as Chin J Microbiol Immunol, 2017, 37(2): 91-96. DOI: 10.3760/cma.j.issn.0254-5101.2017.02.002
Abstract
ObjectiveTo investigate the recognition and binding mechanism between TCR of CD4+ T cells and peptide/MHC (major histocompatibility complex) in immune responses against Mycobacterium tuberculosis (MTB).
MethodsNine patients with tuberculosis (TB) were recruited in the present study. Peptide E7-bound CD4+ T cells were sorted by using E7/HLA-DR tetramers constructed with different HLA-DRB1 alleles from pleural fluid (PLF) of TB patients. Total RNA was extracted and reversely transcribed into cDNA, which was used as the template to amplify complementarity determining region 3 (CDR3) fragments of T cell receptor (TCR) α and β chains. Amino acid sequences, spectratypes and lengths of the amplified CDR3 fragments were analyzed.
ResultsThe CDR3 amino acid sequences of E7-bound CD4+ T cells were identical completely or partially in a single individual. E7/HLA-DR tetramers with different HLA-DRB1 alleles were capable of recognizing and binding CDR3 fragments with identical sequence or similar structure and function in one single individual.
ConclusionThe TCR α and β chains CDR3 lineage of CD4+ T cells of TB patients apparently drifts, and the predominant CDR3 sequences of TCR α and β chains that recognize MTB antigen exhibit peptide specificity with some certain HLA-DR restriction. This study illustrates the possible causes and mechanisms of peptide-specific CD4+ T cells related antigen presentation against MTB.
Key words:
Tuberculosis; E7; HLA-DR tetramer; CDR3; CD4+ T cell
Contributor Information
Yichuan Gan
Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Key Laboratory of Tropical Diseases Control, Ministry of Education, Institute of Tuberculosis, Key Laboratory of Functional Molecules from Marine Microorganisms of Colleges and Universities in Guangdong Province, Guangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Guangzhou 510080, China
Cong Wang
Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Key Laboratory of Tropical Diseases Control, Ministry of Education, Institute of Tuberculosis, Key Laboratory of Functional Molecules from Marine Microorganisms of Colleges and Universities in Guangdong Province, Guangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Guangzhou 510080, China
Yimin Fang
Guangzhou Chest Hospital, State Key Laboratory of Respiratory Disease, Guangzhou 510095, China
Yanan Yao
Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Key Laboratory of Tropical Diseases Control, Ministry of Education, Institute of Tuberculosis, Key Laboratory of Functional Molecules from Marine Microorganisms of Colleges and Universities in Guangdong Province, Guangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Guangzhou 510080, China
Xiaoxin Tu
Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Key Laboratory of Tropical Diseases Control, Ministry of Education, Institute of Tuberculosis, Key Laboratory of Functional Molecules from Marine Microorganisms of Colleges and Universities in Guangdong Province, Guangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Guangzhou 510080, China
Yanming Shen
Guangzhou Chest Hospital, State Key Laboratory of Respiratory Disease, Guangzhou 510095, China
Xiaomin Lai
Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Key Laboratory of Tropical Diseases Control, Ministry of Education, Institute of Tuberculosis, Key Laboratory of Functional Molecules from Marine Microorganisms of Colleges and Universities in Guangdong Province, Guangdong Provincial Research Center for Severe Infectious Disease Prevention and Control Technology, Guangzhou 510080, China
