Bacteriology
Cross immune reaction between Mycobacteria smegmatis and Mycobacteria tuberculosis
Jun Cao, Jinbiao Lu, Anping Xie, Miao Xu, Guozhi Wang, Xiaobing Shen, Baowen Chen, Shuliang Guo
Published 2017-04-30
Cite as Chin J Microbiol Immunol, 2017, 37(4): 275-280. DOI: 10.3760/cma.j.issn.0254-5101.2017.04.007
Abstract
ObjectiveTo identify the cross-reactive antigens shared by Mycobacteria smegmatis(MS) and Mycobacteria tuberculosis(MTB) and to analyze their antigenicity.
MethodsBacterial antigens were extracted from strains of MS and MTB by ultrasonication. Western blot assay was performed to analyze common antigens that reacted with both of the antiserum samples against MS and MTB. The extracted bacterial antigens were mixed with incomplete Freund′s adjuvant and then were injected into muscles of mice. Cytokines secreted by murine spleen lymphocytes following stimulation with various antigens of MS and MTB were determined by ELISPOT and flow cytometry on the 7th day. IgG levels in serum samples were detected by ELISA 7 days after injection.
ResultsThere were cross-reactive antigens shared by MS and MTB. Potent humoral immune responses and cellular immunity against both MS and MTB could be induced by those cross-reactive antigens after sensitization the mice by either MS or MTB antigens. Cytokines of IL-2 and IFN-γ in CD4+ and CD8+ T cells of mice stimulated with MS or MTB antigens were significantly increased as compared with those of non-sensitization group and those of Brucella antigens stimulation group.
ConclusionCross-reactive antigens shared by MS and MTS can effectively promote specific immune reactions to the infection of MTB, which provides a scientific basis for the development of tuberculosis vaccines.
Key words:
Mycobacteria tuberculosis; Mycobacteria smegmatis; Cross-reactive antigen; Immune response
Contributor Information
Jun Cao
Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
Jinbiao Lu
Division of Tuberculosis Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China
Anping Xie
Division of Tuberculosis Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China
Miao Xu
Division of Tuberculosis Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China
Guozhi Wang
Division of Tuberculosis Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China
Xiaobing Shen
Division of Tuberculosis Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China
Baowen Chen
Division of Tuberculosis Vaccines, National Institutes for Food and Drug Control, Beijing 100050, China
Shuliang Guo
Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
