Interleukin-27: a biomarker for prediction of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation from unrelated donors
Shuangzhu Liu, Jingjing Han, Huanle Gong, Jiaqian Qi, Hong Liu, Jia Chen, Shoubao Ma, Depei Wu
Abstract
ObjectiveTo analyze the predictive value of serum interleukin-27 (IL-27) for acute graft-versus-host disease (aGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) from unrelated donors.
MethodsSerum samples were collected from 72 patients after receiving allo-HSCT from unrelated donors during January to December 2012. Serum samples collected from 70 patients received allo-HSCT in 2013 were used for confirmation. All patients received myeloablative conditioning regimen prior to allo-HSCT. Cyclosporin A (CsA)+ mycophenolate mofetil (MMF)+ short-term methotrexate (MTX) were used for GVHD prophylaxis. Serum IL-27 levels in patients with aGVHD were measured by ELISA. The predictive value of IL-27 index, defined as the ratio of serum IL-27 level at neutrophil engraftment to that before pre-conditioning regimen, for allogeneic HSCT was retrospectively analyzed.
ResultsSerum IL-27 index was significantly decreased in patients with grade Ⅱ-Ⅳ aGVHD (grade 0-Ⅰ∶1.89±0.68 vs grade Ⅱ-Ⅳ∶1.26±0.49; P<0.000 1). IL-27 index had good value for grade Ⅱ-Ⅳ aGVHD (AUC=0.782, 95% CI: 0.675-0.889, P<0.001). Patients with a lower serum IL-27 index (<1.33) were more likely to have a higher cumulative incidence of grade Ⅱ-Ⅳ aGVHD than those with a higher serum IL-27 index (P<0.001). Multivariate analysis confirmed that low IL-27 index was the most significant risk factor for grade Ⅱ-Ⅳ aGVHD (HR=4.50, 95% CI: 2.1-9.8, P<0.01). These findings were consistent with the results found in the serum samples collected in 2013.
ConclusionLow IL-27 index could be used to predict the incidence of grade Ⅱ-Ⅳ acute GVHD after allo-HSCT from unrelated donors.
Key words:
Biomarker; Graft-versus-host disease; IL-27; Unrelated donor transplantation
Contributor Information
Shuangzhu Liu
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, China
Institute of Blood and Marrow Transplantation, Soochow University, Suzhou 215213, China
Collaborative Innovation Center of Hematology, Soochow University, Key laboratory of Stem Cells and Biomedical Materials of Jiangsu Province and Chinese Ministry of Science and Technology, Suzhou 215006, China
Jingjing Han
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, China
Institute of Blood and Marrow Transplantation, Soochow University, Suzhou 215213, China
Collaborative Innovation Center of Hematology, Soochow University, Key laboratory of Stem Cells and Biomedical Materials of Jiangsu Province and Chinese Ministry of Science and Technology, Suzhou 215006, China
Huanle Gong
Institute of Blood and Marrow Transplantation, Soochow University, Suzhou 215213, China
Jiaqian Qi
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, China
Institute of Blood and Marrow Transplantation, Soochow University, Suzhou 215213, China
Collaborative Innovation Center of Hematology, Soochow University, Key laboratory of Stem Cells and Biomedical Materials of Jiangsu Province and Chinese Ministry of Science and Technology, Suzhou 215006, China
Hong Liu
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, China
Collaborative Innovation Center of Hematology, Soochow University, Key laboratory of Stem Cells and Biomedical Materials of Jiangsu Province and Chinese Ministry of Science and Technology, Suzhou 215006, China
Jia Chen
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, China
Collaborative Innovation Center of Hematology, Soochow University, Key laboratory of Stem Cells and Biomedical Materials of Jiangsu Province and Chinese Ministry of Science and Technology, Suzhou 215006, China
Shoubao Ma
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, China
Institute of Blood and Marrow Transplantation, Soochow University, Suzhou 215213, China
Collaborative Innovation Center of Hematology, Soochow University, Key laboratory of Stem Cells and Biomedical Materials of Jiangsu Province and Chinese Ministry of Science and Technology, Suzhou 215006, China
Depei Wu
Jiangsu Institute of Hematology, the First Affiliated Hospital of Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, China
Institute of Blood and Marrow Transplantation, Soochow University, Suzhou 215213, China
Collaborative Innovation Center of Hematology, Soochow University, Key laboratory of Stem Cells and Biomedical Materials of Jiangsu Province and Chinese Ministry of Science and Technology, Suzhou 215006, China
