Virology
Serotype distribution of enteroviruses in patients with influenza-like illness in Beijing
Lingyu Shen, Jiaxin Du, Fangyao Liu, Hongbo Jing, Cheng Gong, Ming Luo, Fang Huang, Tiegang Zhang
Published 2018-03-30
Cite as Chin J Microbiol Immunol, 2018, 38(3): 176-180. DOI: 10.3760/cma.j.issn.0254-5101.2018.03.003
Abstract
ObjectiveTo analyze the serotypes of enteroviruses (EVs) isolated from patients with influenza-like illness in Beijing in 2017.
MethodsOropharyngeal swab specimens were collected from patients with influenza-like illness in eight districts of Beijing from July 2017 to October 2017. EVs were detected by real-time PCR. Specific primers were synthesized and used to amplify the VP1 fragments of EVs. PCR products were sequenced and the results were compared with the reference sequences by using Basic Local Alignment Search Tool (BLAST) to identify the serotypes of isolated EVs.
ResultsA total of 666 specimens were collected and 91 (13.66%) were positive for EVs. VP1 sequences of 66 EVs were successfully amplified and BLAST analysis revealed that these strains belonged to 14 serotypes, including seven serotypes of EV-A species, six serotypes of EV-B species and one serotype of Rhinovirus species. The predominant serotypes were CVA2 and CVA6. Eight out of 14 CVA6 strains that were collected in Shunyi District shared high homology. All seven CVB5 strains were collected in Shijingshan District and grouped into one cluster.
ConclusionEVs causing influenza-like illness in Beijing in 2017 belonged to 14 serotypes and CVA2 and CVA6 were the predominant serotypes.
Key words:
Enterovirus; Influenza-like illness; Serotype
Contributor Information
Lingyu Shen
School of Public Health, Capital Medical University, Beijing 100069, China
Jiaxin Du
Dongcheng District Center for Disease Control and Prevention, Beijing 100005, China
Fangyao Liu
Haidian District Center for Disease Control and Prevention, Beijing 100095, China
Hongbo Jing
Shunyi District Center for Disease Control and Prevention, Beijing 101300, China
Cheng Gong
Beijing Center for Diseases Prevention and Control, Beijing Preventive Medicine Research Center, Beijing 100013, China
Ming Luo
Beijing Center for Diseases Prevention and Control, Beijing Preventive Medicine Research Center, Beijing 100013, China
Fang Huang
Beijing Center for Diseases Prevention and Control, Beijing Preventive Medicine Research Center, Beijing 100013, China
Tiegang Zhang
Beijing Center for Diseases Prevention and Control, Beijing Preventive Medicine Research Center, Beijing 100013, China
