Microbiology
Classification and phylogenetic analysis of the genus Mobiluncus based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and whole genome sequencing
Xueying Zhang, Hong Ye, Aiyun Li, Guangyong Ye, Nan Feng, Yu Ma, Yuning Zhu, Yingbo Shen, Rong Zhang
Published 2018-06-30
Cite as Chin J Microbiol Immunol, 2018, 38(6): 446-450. DOI: 10.3760/cma.j.issn.0254-5101.2018.06.008
Abstract
ObjectiveTo identify Mobiluncus species with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 16S rRNA gene sequencing and to analyze antibiotic resistance genes and phylogenetic relationships among Mobiluncus species with whole genome sequencing.
MethodsTwenty-five Mobiluncus strains were isolated after anaerobic culture of 65 vaginal secretion samples of patients with bacterial vaginosis (BV) and identified to species level by MALDI-TOF MS and 16S rRNA gene sequencing. The whole genome DNA of each strain was extracted for next-generation sequencing. SPAdes was used to assembly genomes. Resistance genes were searched in ARGD database. The core genome of all isolates was analyzed by Harvest to construct phylogenetic tree.
ResultsMALDI-TOF MS could only identify Mobiluncus curtisii, while 16S rRNA gene sequencing could identify both Mobiluncus mulieris and Mobiluncus curtisii. Results of the whole genome sequencing showed that tetracycline resistance gene tet(o) and macrolides resistance gene erm(x) were the two predominant acquired resistance genes of Mobiluncus with a positive rate of 84.7% and 61.5% respectively. Intra-species relationships of the two Mobiluncus species were close, but a distant phylogenetic relationship was found between the two species.
ConclusionThis study shows that MALDI-TOF MS can′t be used to identify Mobiluncus mulieris at present. Mobiluncus strains have potential resistance to tetracycline and macrolides. Intra-species evolution of Mobiluncus is slow, which indicates that there is no growing trend towards new species for the time being.
Key words:
Mobiluncus; Bacterial vaginosis (BV); MALDI-TOF MS; Whole genome sequencing; Resistance gene
Contributor Information
Xueying Zhang
Department of Clinical Laboratory, Women′s Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China
Hong Ye
Department of Clinical Laboratory, Women′s Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China
Aiyun Li
Department of Clinical Laboratory, Women′s Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China
Guangyong Ye
Department of Clinical Laboratory, Women′s Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China
Nan Feng
Department of Clinical Laboratory, Women′s Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China
Yu Ma
Department of Clinical Laboratory, Women′s Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China
Yuning Zhu
Department of Clinical Laboratory, Women′s Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China
Yingbo Shen
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
Rong Zhang
Department of Clinical Laboratory, the Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China
