Basic Immunology
Detection of peripheral CD14+ CD277+ monocyte-macrophage ratio with γδ TCR tetramer and analysis of its relationship with treatment outcome
Mao Ling, Mei Zhixiong, Tu Xiaoxin, Fang Yimin, Gan Yichuan, Shen Yanming, Lai Xiaomin
Published 2018-11-30
Cite as Chin J Microbiol Immunol, 2018,38(11): 801-806. DOI: 10.3760/cma.j.issn.0254-5101.2018.11.001
Abstract
ObjectiveTo investigate the role of peripheral CD14+ monocyte-macrophages in the recognition of phosphorylated antigen by γδ T cells and its relationship with treatment outcome.
MethodsThree kinds of γδ TCR tetramers were used to stain PBMC collected from patients with tuberculosis (TB) and neonatal umbilical cord blood samples. The proportions of various TB-specific antigen presenting cells (APC) in peripheral blood were analyzed, and their relationships with treatment outcome were assessed based upon clinical data.
ResultsCD14+ monocyte-macrophages both in tuberculosis patients′ peripheral blood and neonatal umbilical cord blood were the strongest binding cells to CD277 antibody and γδ TCR tetramers. The median (P50) of CD14+ monocyte-macrophages reached the highest peak after taking anti-tuberculosis treatment for about one month and patients′ condition was improved obviously during this period.
ConclusionThis study elucidated that CD14+ monocyte-macrophages accounted for the largest proportion of APC when γδ T cells recognized phosphorylated antigens, which provided reference data for further study on the mechanism of γδ T cells restrictively recognizing phosphorylated antigen and their significance in innate and adaptive immunity.
Key words:
Tuberculosis; γδTCR tetramer; Flow cytometry; Monocyte-macrophage; Treatment outcome
Contributor Information
Mao Ling
Department of Microbiology, Zhongshan School of Medicine, Key Laboratory of the Ministry of Education for Tropical Disease Prevention and Control, Institute of Tuberculosis, Guangdong Key Laboratory of Colleges of Marine Microbial Functional Molecules, Guangdong Provincial Research Center for Severe Infectious Disease Prevention and Control, Sun Yat-sen University, Guangzhou 510080, China
Mei Zhixiong
the Third Affiliated Hospital (Lingnan Hospital), Sun Yat-sen University, Guangzhou 510530, China
Tu Xiaoxin
Department of Microbiology, Zhongshan School of Medicine, Key Laboratory of the Ministry of Education for Tropical Disease Prevention and Control, Institute of Tuberculosis, Guangdong Key Laboratory of Colleges of Marine Microbial Functional Molecules, Guangdong Provincial Research Center for Severe Infectious Disease Prevention and Control, Sun Yat-sen University, Guangzhou 510080, China
Fang Yimin
State Key Laboratory of Respiratory Disease, Guangzhou Chest Hospital, Guangzhou 510095, China
Gan Yichuan
Department of Microbiology, Zhongshan School of Medicine, Key Laboratory of the Ministry of Education for Tropical Disease Prevention and Control, Institute of Tuberculosis, Guangdong Key Laboratory of Colleges of Marine Microbial Functional Molecules, Guangdong Provincial Research Center for Severe Infectious Disease Prevention and Control, Sun Yat-sen University, Guangzhou 510080, China
Shen Yanming
State Key Laboratory of Respiratory Disease, Guangzhou Chest Hospital, Guangzhou 510095, China
Lai Xiaomin
Department of Microbiology, Zhongshan School of Medicine, Key Laboratory of the Ministry of Education for Tropical Disease Prevention and Control, Institute of Tuberculosis, Guangdong Key Laboratory of Colleges of Marine Microbial Functional Molecules, Guangdong Provincial Research Center for Severe Infectious Disease Prevention and Control, Sun Yat-sen University, Guangzhou 510080, China
