Expression and clinical significance of novel protein kinase C ε in prostate cancer tissuss
HUANG Bin, CHEN Jun-xing, ZHAO Liang-yun, MAO Xiao-peng, QIU Shao-peng
Published 2011-09-15
Cite as Chin J Urol, 2011,32(09): 607-609. DOI: 10.3760/cma.j.issn.1000-6702.2011.09.008
Abstract
Objective To explore the expression of novel protein kinase C ε (PKCε) in normal prostate (NP) tissue, benign prostate hyperplasia(BPH), peficancerous (PC) tissue and prostate cancer (Pca), and study its correlation with the grade and stage of Pca.MethodsTen NP slides, ten BPH slides, ten PC slides and 43 Pca slides were collected from our hospital. These slides were routinely proceased and analyzed according to the requirement of immunohistochemical staining. Tumors were classified according to the 2002 TNM staging system. The grading system used in the study was based on the Gleason grade.Results We was found that the expression of PKCεs in Pca (27/43) were significantly higher than those in NP(1/10), BPH (0/10) and PC (2/10) tissue, and the difference was statistically significant ( P <0.05 ). With regard to grade of prostate cancer, the expression of PKCε in Pca with Gleason score ≥8 group (12/13) was higher than the Gleason score 2 -4 group (4/10) and the Gleason score 5 -7 group (11/20). The difference was statistically significant (P < 0.05 ). Moreover, the T3 and T4 stages had a more positive rate (10/12 & 9/10) than the T1 and T2 stages( 1/6 &7/15). There is statistically significant difference between early and advanced stage of prostate cancer ( P < 0. 05 ). Furthermore, the positive expression of PKCε in prostatic carcinoma samples increased significantly in the metastasis group (9/10)compared to the non-metastasis group ( 18/33 ) ( P < 0. 05 ), but the difference was not statistically significant between the concentration of prostate-specific antigen in blood serum ( P > 0. 05 ).Conclusions PKCε is expressed in prostate cancer, and it correlates with the grade and stage of prostate cancer. PKCε may be related to the origin and the development of Pca, and it may be used as a prognostic factor for Pca.
Key words:
Prostate cancer; Protein kinase Cε ; Immunohistochemistry
Contributor Information
HUANG Bin
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
CHEN Jun-xing
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
ZHAO Liang-yun
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
MAO Xiao-peng
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China
QIU Shao-peng
Department of Urology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China