Immunohistochemical analysis of primary renal cell carcinomas and paired bone metastases
Zhang Lijie, Huang Xiaobo, Xu Tao, Xu Qingquan, Wang Xiaofeng
Published 2014-08-15
Cite as Chin J Urol, 2014,35(08): 561-564. DOI: 10.3760/cma.j.issn.1000-6702.2014.08.001
Abstract
Objective To compare the histopathologic and immunohistochemical differences between primary renal cell carcinomas and paired bone metastases in order to discuss the significance in the selection of standard targeted therapies.Methods The clinical data of 19 patients who underwent nephrectomy and resection of bone metastases successively from January 2003 to September 2013 were analysed retrospectively.The paraffin-embedded surgical samples of all the patients were obtained for histopathologic and immunohistochemical analysis.The differences of Fuhrman grades,expression of Ki-67,CD34,vascular endothelial growth factor receptor 2 (VEGFR2),epidermal growth factor receptor (EGFR) and CXC subfamily receptor 4 (CXCR4) were compared between primary renal cell carcinomas and their paired bone metastases.Microvessel density (MVD) was evaluated by the CD34 immunostaining.Results The Fuhrman grades of samples from bone metastases were higher than that of primary tumors (36.8%,7/19) (P=0.008).The Ki-67 label index was (4.00±3.96)% in primary tumors and (7.90±7.38)% in bone metastases (P=0.033).The microvessel density (MVD) was 58.13±22.90 in primary tumors and 46.71±25.40 in the bone metastases (P=0.026).The immunohistochemistry scores of VEGFR2 were 4.68±1.20 in primary tumors and 4.05±1.58 in bone metastases (P=0.014).The immunohistochemistry scores of EGFR were 5.89±1.05 in primary tumors and 5.47± 1.12 in bone metastases (P=0.134).The immunohistochemistryscores of CXCR4 in cytomembrane and cytoplasm were 1.74±1.97 in primary tumors and 2.16± 1.64 in bone metastases (P=0.414).The inununohistochemistry scores of CXCR4 in cell nucleus were 2.52±2.09 in primary tumors and 3.42±1.95 in bone metastases (P=0.009).Conclusions The Fuhrman grades and the expression of Ki-67 and CXCR4 in cell nucleus were higher in bone metastases than that in the primary renal cell carcinomas.The MVD and the expression of VEGFR2 were lower in bone metastases than that in the primary tumors.The above alternations may contribute to the poor prognosis of bone metastasis and the poor result of angiosuppressive therapy.
Key words:
Carcinoma, renal cell; Immunohistochemistry; Neoplasm metastasis; Molecular targeted therapy
Contributor Information
Zhang Lijie
Department of Urology, Peking University People's Hospital, Beijing 100044, China
Huang Xiaobo
Department of Urology, Peking University People's Hospital, Beijing 100044, China
Xu Tao
Department of Urology, Peking University People's Hospital, Beijing 100044, China
Xu Qingquan
Department of Urology, Peking University People's Hospital, Beijing 100044, China
Wang Xiaofeng
Department of Urology, Peking University People's Hospital, Beijing 100044, China