Basic Research Article
Analysis of the MicroRNA expression profiles in the kidney of calcium oxalate stones rats
Kang Chen, Chuangxin Lan, Dong Yang, Chao Cai, Xiaolu Duan, Shaohong Xu, Yang Liu, Dong Chen, Xin Mai, Wenqi Wu, Guohua Zeng
Published 2016-05-15
Cite as Chin J Urol, 2016, 37(5): 380-384. DOI: 10.3760/cma.j.issn.1000-6702.2016.05.016
Abstract
ObjectiveTo compare the miRNA expression profiles in kidney tissues of normal and calcium oxalate stone rats, and to explore the roles of different miRNAs in the regulation of kidney stone formation.
MethodsSixteen 6-8 weeks old SD rats were divided into two groups, including the normal group and the model group randomly, between Sep 2015 and Dec 2015. The normal group was permitted to drink water freely; whereas the model of calcium oxalate stone group was established by freely drinking water with 1% ethylene glycol and 1% ammonium chloride. 28 days after molding, miRNA microarray and mRNA microarray were applied to evaluate the miRNA expressions in the kidney tissues of both normal and calcium oxalate stone rats. Target prediction, Gene Ontology (GO) analysis , Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were applied to predict the potential roles of miRNAs in the biological processes of stone formation.
ResultsThere were many crystals in calcium oxalate stone rat's kidney tissue sections whereas no crystal was detected in normal group. 38 miRNAs were differentially expressed with a more than 2-fold changing (P<0.05) between normal and calcium oxalate stone rats. Among those miRNAs, 18 miRNAs were up-regulated, while 20 miRNAs were down-regulated. 2 728 mRNAs were differentially expressed with a more than 2-fold change (P<0.05) between the two groups. Among them, 1 350 mRNAs were up-regulated and 1 378 were down-regulated. Gene Ontology analysis suggested that those differentially expressed miRNAs targeted many genes which were related to the function of calcium ion transport, injury, inflammatory response, oxidative stress and cell adhesion. KEGG pathway analysis suggested that those miRNAs were involved in inflammation-related pathway including cytokine-cytokine receptor interaction and chemokine signaling pathway.
ConclusionsThere were significantly differences in miRNA expression profiles between normal and calcium oxalate stone rats, and these differentially expressed miRNAs might be associated with the function of regulation of calcium ion transport, inflammatory response, response to wounding and cell adhesion in the biological processes of calcium oxalate stone formation.
Key words:
Calcium oxalate stones; Microarray; MicroRNA expression profiles
Contributor Information
Kang Chen
Department of Urology, First Affiliated Hospital of Guangzhou Medical University, Guangdong Key Laboratory of Urology, Guangzhou 510230, China
Chuangxin Lan
Dong Yang
Chao Cai
Xiaolu Duan
Shaohong Xu
Yang Liu
Dong Chen
Xin Mai
Wenqi Wu
Guohua Zeng
