Clinical Original Article
Characteristics of urinary microflora in women with type 2 diabetic peripheral neuropathy without lower urinary tract symptoms
Li Ying, Gao Fang, Chen Jiawei, Fan Xinzhao, Zhan Wanhua, Wu Peng, Xue Yaoming, Cao Ying
Published 2020-03-15
Cite as Chin J Urol, 2020,41(03): 219-224. DOI: 10.3760/cma.j.cn112330-20190923-00420
Abstract
ObjectiveTo investigate the characteristics of urinary microflora in women with type 2 diabetic peripheral neuropathy without lower urinary tract symptoms.
MethodsBy completing nerve conduction function and the American Urological Association Symptom Index questionnaire (AUA-SI), a total of 30 cases of women hospitalized with type 2 diabetes and no symptoms of lower urinary tract from May 2017 to August 2018 were included. 17 patients with diabetic peripheral neuropathy were assigned to the DPN group, and 13 patients without diabetic peripheral neuropathy were assigned to the nDPN group. Urine specimens were collected from clean catch midstream urine and processed for extracting DNA. Microbial diversity and composition were analyzed using the Illumina sequencing platform targeting to 16S rDNA gene. Sequencing reads were processed by QIIME. LEfSe algorithm was used to analyze the flora with significant differences between the two groups.
ResultsThe duration of diabetes in the DPN group was lower than that in the nDPN group [(4.12 ± 3.28)years vs.(8.03 ± 6.11)years, P = 0.03], and the retinopathy cases were more in the DPN group than those in the nDPN group (6 vs. 0, P=0.03). Except for above two indicators, there was no significant difference in demographic characteristics between DPN group and nDPN group(P>0.05). The urinary microenvironment of DPN was characterized by increased bacterial richness(sobs index, chao index and aec index, 67.24±40.25 vs.108.69±57.18; 81.36±47.99 vs.122.55±55.70; 88.58±55.03 vs.125.78±53.03, all P<0.05) and by the enrichment of Mycoplasmataceae(Metastats value: 0.52±0.01vs.0.01±0.00001, P=0.02). Beta diversity showed that no significant difference of bacterial composition was found between these two group(P>0.05). LEfSe analysis showed that at the genus level, the relative abundance of eight genera(e.g., Bacillus, Duganella, Leptotrichia, Proteus, Propionibacterium, Pseudoxanthomonas, Bdellovibrio and uncultured_soil_bacterium) in DPN group decreased at the level of genus(P<0.05).
ConclusionsFemale patients with type 2 diabetes without lower urinary tract symptoms of peripheral neuropathy exhibit a different microbial community compared to nDPN controls. Mycoplasmataceae may be a potential biomarker for patients with DPN.
Key words:
Diabetic peripheral neuropathy; No lower urinary tract symptoms; Urinary microbiome
Contributor Information
Li Ying
Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Gao Fang
Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Chen Jiawei
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Fan Xinzhao
Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Zhan Wanhua
Department of Endocrinology and Metabolism, Zengcheng Branch of Nanfang Hospital, Southern Medical University, Guangzhou 511300, China
Wu Peng
Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Xue Yaoming
Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Cao Ying
Department of Endocrinology and Metabolism, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
