Clinical Investigation
The temporal profile of astrocytes and Jak-STAT signal pathway after spinal ischemia and reperfusion injury in rabbits
Yunlu Wang, Lei Tian, Shiyao Liu, Zhigao Ma, Siyu Hou, Yanwei Yang, Huixian Li, Mu Jin, Xiuhua Dong, Jiakai Lu, Weiping Cheng
Published 2018-02-25
Cite as Chin J Thorac Cardiovasc Surg, 2018, 34(2): 104-109. DOI: 10.3760/cma.j.issn.1001-4497.2018.02.011
Abstract
ObjectiveTo observe the evolution of astrocytes, GDNF, BDNF and Jak-STAT signal pathway after spinal cord ischemia-reperfusion injury in rabbits.
MethodsSpinal cord ischemia was induced by means of balloon occlusion of the infrarenal aorta for 22 minutes in 54 male New Zealand white rabbits. We assigned rabbits to 9 groups(n=6), one sham group, eight operation groups. The operation process in the sham group was the same as the operation group except the ischemia reperfusion of the spinal cord. At 0 h, 1 h, 2 h, 3 h, 8 h, 24 h, 48 h and 72 h after reperfusion, animals were sarcrificed and the spinal cord was removed for histologic, immunohistochemical study and western blotting.
ResultsNormal neurons were decreased with the extension of reperfusion time. Levels of GFAP increased at 3 h and reached a peak at 48 h after reperfusion. GDNF was increased reaching two peaks after injury, the first peak was at 3 h, the second was at 72 h. BDNF level was increased and peaked at 24 h after reperfusion. The expression of p-STAT3 showed a biphasic pattern which peaked at 1h and 48 h. GFAP, GDNF, BDNF were rare and the level of p-STAT3 could be neglected in sham group.
ConclusionSpinal cord ischemia-reperfusion injury could induce the activation of astrocytes, the expression of GDNF, BDNF and the activation of Jak-STAT signal pathway. They showed different expression rules in this study.
Key words:
Spinal cord; Injury, ischemia-reperfusion; Astrocytes; GDNF; BDNF; Jak-STAT signaling pathway
Contributor Information
Yunlu Wang
Department of Anesthesiology, Beijing Anzhen Hospital, Capital Medical University and Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing 100029, China
Lei Tian
Shiyao Liu
Zhigao Ma
Siyu Hou
Yanwei Yang
Huixian Li
Mu Jin
Xiuhua Dong
Jiakai Lu
Weiping Cheng