Basic Research
Selection of the appropriate pore size, surface modification technique for 3D printed tracheal graft and evaluation of its property
Yibo Shan, Yiwei Fan, Jianfeng Li, Hongcan Shi, Shu Pan, Fangbiao Zhang
Published 2019-03-25
Cite as Chin J Thorac Cardiovasc Surg, 2019, 35(3): 171-176. DOI: 10.3760/cma.j.issn.1001-4497.2019.03.011
Abstract
ObjectiveTo prepare 3D printed porous tracheal graft fabricated by PCL and to select the appropriate pore size and surface modification techniques, in order to explore its effect on cell behavior.
MethodsThe PCL porous tracheal graft was prepared by 3D printing technology and biomechanical properties of the graft were measured by means of longitudinal tension, radial compression and three-point bending test. The porous grafts were surface-modified through hydrolysis, amination and nanocrystallization treatment and then characterized by energy dispersive spectroscopy(EDS). The effect of different pore sizes and surface modifications on the cell proliferation behavior was evaluated by CCK-8 and scanning electron microscopy(SEM).
ResultsThe 3D printed porous tracheal graft had similar morphology with the native tracheas(P>0.05) and better biomechanical properties(P<0.05). It was more suitable for cell adhesion and proliferation when the pore size is 200 μm(P<0.05). Compared to hydrolysis and amination, nanocrystallization treatment successfully improved the cytotropism of the 3D printed tracheal graft(P<0.05).
Conclusion3D printed porous tracheal graft shows favorable biomechanical properties. The appropriate pore size of the 3D printed porous tracheal graft is 200 μm and the appropriate surface modification techniques is nanocrystallization.
Key words:
3D printing Trachea Polycaprolactone Surface modifications Evaluation
Contributor Information
Yibo Shan
Medical College of Yangzhou University, Yangzhou 225001, China
Jiangyin People’s Hospital, Jiangyin 214400, China
Center of translational medicine of Yangzhou University, Yangzhou 225001, China
Yiwei Fan
Medical College of Yangzhou University, Yangzhou 225001, China
Center of translational medicine of Yangzhou University, Yangzhou 225001, China
Jianfeng Li
Medical College of Yangzhou University, Yangzhou 225001, China
Center of translational medicine of Yangzhou University, Yangzhou 225001, China
Hongcan Shi
Medical College of Yangzhou University, Yangzhou 225001, China
Center of translational medicine of Yangzhou University, Yangzhou 225001, China
Shu Pan
Medical College of Yangzhou University, Yangzhou 225001, China
Center of translational medicine of Yangzhou University, Yangzhou 225001, China
Fangbiao Zhang
Department of Cardiothoracic Surgery, the Central Hospital of Lishui City, Lishui 323000, China
