Preparation of internal quality control substance of real-time RT-PCR to quantitative influenza virus RNA and drawing quality control chart
Liu Liqi, Zhao Xiang, Wen Leying, Guo Junfeng, Wei Hejiang, Wang Dayan, Shu Yuelong
Published 2014-01-30
Cite as Chin J Exp Clin Virol, 2014,28(01): 55-57. DOI: 10.3760/cma.j.issn.1003-9279.2014.01.019
Abstract
Objective To draw a Levey-Jennings quality control chart using influenza virus RNA as internal quality control substance.Method Recent popular representative strains of influenza virus were selected and extracted RNA.Make series 10 folds dilution and take different dilution to do quantitative RTPCR.Three parallel wells of each dilution were done.High Ct value and low Ct value corresponding dilution of RNA were selected and detected continuously twenty times.Mean Ct value (x) and standard deviation (s) were calculated.Levey-Jennings quality control chart were drew by using Excel software.Result Levey-Jennings quality control charts of three influenza virus subtype were drew and each internal quality control substance was in control.Conclusion RNA quality substance of each subtype has good stability and preparation simple which is convenient to draw quality control chart and suitable as internal quality control substance for PCR laboratory.
Key words:
Influenza A virus; RNA; Quality control
Contributor Information
Liu Liqi
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China
Zhao Xiang
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China
Wen Leying
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China
Guo Junfeng
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China
Wei Hejiang
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China
Wang Dayan
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China
Shu Yuelong
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Beijing 102206, China
