Objective To investigate the expression profile of microRNA in endometrioid adenocarcinoma.Methods The microRNA chips were used to detect the different microRNAs in 3 pairs of endometrioid adenocarcinoma and adjacent nontumorous endometrium,and the expressions of five miRNAs were validated using real time stem-loop reverse transcription-PCR.Results 68 miRNAs exhibited higher expression and 81 miRNAs exhibited lower expression in endometrioid adenocarcinoma samples than those in the nontumorous samples in the microarray,and the results were further confirmed by detecting the higher expressing microRNAs including hsa-miR-205,hsa-miR-200b and hsa-miR200c,and lower expressing microRNAs including hsa-miR-495 and hsa-miR-216b using real time PCR.Conclusions:The microRNA profile may play critical roles in tumorigenesis of endometrioid adenocarcinoma and serve as a diagnostic or therapeutic tool in endometrioid adenocarcinoma future.