Original Article
Effects of different antigen stimulation methods on the proliferation of HIV specific CD8 T cells
Cong Jin, Ting Tu, Danlei Mou, Qunhui Li, Tao Li, Hao Wu, Huabiao Chen
Published 2016-06-30
Cite as Chinese J Exp Clin Virol, 2016, 30(3): 257-262. DOI: 10.3760/cma.j.issn.1003-9279.2016.03.001
Abstract
ObjectiveTo study the factors that influence the ex vivo proliferation of human immunodeficiency virus (HIV) specific CD8 T cells.
MethodsThree methods to use HIV-specific antigen peptides stimulating the proliferation of virus specific CD8 T cells from HIV patients were compared, and the effect of adding exogenous IL-2 into co-culture on proliferation was evaluated.
ResultsIn comparison to directly adding MHC I matched HIV peptides into peripheral blood mononuclear cells (PBMCs) by presence during co-culture or incubating for 2 hours, the more efficient proliferation of HIV-specific CD8 T cells was stimulated by loading HIV peptides on the mixed antigen presenting cells (APCs) that harvested by removing CD8 T cells from PBMCs. Adding IL-2 into co-culture could largely enhance the number of proliferated HIV-specific CD8 T cells but also induced non-specific proliferation of CD8 T cells.
ConclusionsStimulation of HIV-specific CD8 T cells by loading HIV-specific antigen peptides on mixed APCs and in absence of IL-2 can specifically induce the efficient proliferation of HIV-specific CD8 T cells by providing both antigen and co-stimulation signals.
Key words:
Acquired immunodeficiency syndrome; CD8 T cells; Ex vivo proliferation
Contributor Information
Cong Jin
National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Ting Tu
Jiangsu Key Laboratory of Clinical Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang 212013, China
Danlei Mou
Beijing You′an Hospital Affilited Capital Medical University, Beijing 100069, China
Qunhui Li
Beijing You′an Hospital Affilited Capital Medical University, Beijing 100069, China
Tao Li
Jiangsu Key Laboratory of Clinical Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang 212013, China
Hao Wu
Beijing You′an Hospital Affilited Capital Medical University, Beijing 100069, China
Huabiao Chen
Jiangsu Key Laboratory of Clinical Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang 212013, China
Massachusetts General Hospital, USA
