Original Article
Typing analysis and detection of HPV for condyloma acuminata tissues of perianal
Zhaojie Xu, Yewei Deng, Rui Li
Published 2017-02-28
Cite as Chinese J Exp Clin Virol, 2017, 31(1): 35-37. DOI: 10.3760/cma.j.issn.1003-9279.2017.01.007
Abstract
ObjectiveTo learn the prevalence of HPV infection and its genotypes distribution in perianal warts, discuss the association between HPV infection and age and gender, and assess the methodology performance of PCR-reverse dot blot(PCR-RDB) to detect HPV.
MethodsHPV genotypes were detected in 316 paraffin specimens of confirmed or suspected perianal condyloma acuminata(CA) patients, including 58 cases for HPV DNA sequencing.
ResultsThe HPV infection rate in 316 patients was 81.6%. The first fifth genotypes of single HPV infection were HPV11, 6, 18, 16 and 33. HPV6+ 11, HPV6+ 11+ 16 and HPV6+ 18 were more common in mixed infection. The highest HPV infection rate was from the males aged below 35 years. Regarding the sequencing result as a gold standard, the sensitivity, specificity and accuracy of PCR-RDB in HPV were 92.31%, 89.47% and 91.38% respectively.
ConclusionsThe proportion of HPV crissum infection in young male was quite high, the major pathogenic types were HPV 11, 6, 18, 16 and 33. The PCR-RDB method can meet the needs of clinical diagnosis of HPV.
Key words:
Papillomavirus human; Genotyping; Condyloma Acuminata
Contributor Information
Zhaojie Xu
Department of Colorectal, Zhengzhou People＇s Hospital Affiliated to Southern Medical University, Zhengzhou 450000, China
Yewei Deng
Department of Colorectal, Zhengzhou People＇s Hospital Affiliated to Southern Medical University, Zhengzhou 450000, China
Rui Li
Clinical Laboratory, Zhengzhou Children＇s Hospital, Zhengzhou 450053, China
