Original Article
Characteristics of the human adenovirus infections from influenza-like illness samples in Xi′an
Xu Jing, Yue Shuting, Ma Ping, Zhang Lei, Shi Wei, Xu Yi, Li Shen, Guan Luyuan, Wang Jingjun, Yu Pengbo
Published 2017-06-30
Cite as Chin J Exp Clin Virol, 2017,31(03): 218-222. DOI: 10.3760/cma.j.issn.1003-9279.2017.03.008
Abstract
ObjectiveTo analyze the epidemiological characteristics and the molecular types of human adenovirus (HAdV) from influenza-like illness (ILI) samples with negative influenza virus in Xi'an from January 2013 to December 2015.
MethodsSamples from patients with ILI were collected from two national influenza sentinel surveillance hospitals during 2013—2015 in Xi′an. HAdV was detected by real-time PCR, and then the positive samples were inoculated into Hep-2 cells to isolate the viruses. The amplified products were purified and sequenced of hexon gene, and the sequences were compared with the Genebank data and phylogenetic trees were constructed.
ResultsIn 2367 samples, 88 samples were positive for HAdV, the positive rate was 3.72%. There were 7 subtypes detected, and the rates of each subtype are as follows: HAdV-1 was 9.09%, HAdV-2 was 22.73%, HAdV-3 was 23.86%, HAdV-4 was 5.68%, HAdV-5 was 7.95%, HAdV-6 was 3.41% and HAdV-7 was 1.14%. Males had higher infection rate than females, but there was no significant difference. The patients were divided into 6 groups according to age. There were 3 positive samples among those under 1 year of age, 36 positive samples among those 1 to 3 years old, 26 positive samples among those 4 to 6 years old, 16 positive samples in those 7 to 18 years old, 5 positive samples in 19 to 59 years old and 2 positive samples in those older than sixty years of age. HAdV infection was primarily confined to children under 7 years of age.
ConclusionsHAdV-3 and HAdV-2 were the dominant epidemic strains during 2013—2015 in Xi′an. Children younger than 7 years were the main susceptible population. HAdV infections circulate all year-round and there was no considerable seasonal variation.
Key words:
Human Adenovirus; Epidemiological characteristics; Molecular typing
Contributor Information
Xu Jing
Viroloy Research Laboratory of Shaanxi Provincial Center for Disease Control and Prevention, Xi＇an 710054, China
Yue Shuting
School of Public Health, Xi′an Jiaotong University Health Science Center, Xi′an 710061, China
Ma Ping
Viroloy Research Laboratory of Shaanxi Provincial Center for Disease Control and Prevention, Xi＇an 710054, China
Zhang Lei
Viroloy Research Laboratory of Shaanxi Provincial Center for Disease Control and Prevention, Xi＇an 710054, China
Shi Wei
Viroloy Research Laboratory of Shaanxi Provincial Center for Disease Control and Prevention, Xi＇an 710054, China
Xu Yi
Viroloy Research Laboratory of Shaanxi Provincial Center for Disease Control and Prevention, Xi＇an 710054, China
Li Shen
Viroloy Research Laboratory of Shaanxi Provincial Center for Disease Control and Prevention, Xi＇an 710054, China
Guan Luyuan
Viroloy Research Laboratory of Shaanxi Provincial Center for Disease Control and Prevention, Xi＇an 710054, China
Wang Jingjun
Viroloy Research Laboratory of Shaanxi Provincial Center for Disease Control and Prevention, Xi＇an 710054, China
Yu Pengbo
Viroloy Research Laboratory of Shaanxi Provincial Center for Disease Control and Prevention, Xi＇an 710054, China
