Original Article
Investigation of mosquitoes and arboviruses in the border areas of Yunnan province, 2012
Wenwen Lei, Xiaofang Guo, Shihong Fu, Yun Feng, Zhonghua Yang, Huanyu Wang, Ying He, Xiaoyan Gao, Zhi Lyu, Hongning Zhou, Guodong Liang
Published 2017-08-30
Cite as Chinese J Exp Clin Virol, 2017, 31(4): 311-314. DOI: 10.3760/cma.j.issn.1003-9279.2017.04.008
Abstract
ObjectiveTo investigate the distribution patterns of arboviruses in Yunnan province near the China-Laos-Myanmar border, China, and to provide evidence for prevention and control of arboviruses diseases.
MethodsMosquito samples were collected in Daluo county of Xishuangbanna Dai Autonomous Prefecture and Zhengdong county of Pu’er city in Yunnan province, 2012. Viruses were isolated from the samples by tissue culture, positive isolates were identified by RT-PCR with arbovirus species-specific primers, for further sequencing and phylogenetic analysis.
ResultsA total of 17 species of mosquitoes from 6 genera were collected. A total of 24 strains of viruses were isolated from the mosquito pools and identified as Tembusu virus (TMUV) (2 strains), Japanese encephalitis virus (JEV) (3 strains), Getah virus (GETV) (2 strains), Banna virus (BAV) (4 strains), Densovirus (DNV) (9 strains) and Nam Dinh virus (NDiV) (3 strains).
ConclusionsThe China-Laos-Myanmar border of Yunnan province is rich in species of mosquitoes and arboviruses.
Key words:
Arbovirus; Yunnan province; Investigation
Contributor Information
Wenwen Lei
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xiaofang Guo
Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Institute of Parasitic Diseases, Pu’er 665000, China
Shihong Fu
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Yun Feng
Yunnan Institute of Endemic Disease Control and Prevention, Yunnan Provincial Center of Virus and Rickettsia Research, Dali 671000, China
Zhonghua Yang
Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Institute of Parasitic Diseases, Pu’er 665000, China
Huanyu Wang
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Ying He
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xiaoyan Gao
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Zhi Lyu
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Hongning Zhou
Yunnan Provincial Key Laboratory of Vector-borne Diseases Control and Research, Yunnan Institute of Parasitic Diseases, Pu’er 665000, China
Guodong Liang
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
