Original Article
Isolation and identification of Getah virus from Culicoides in Yunnan
Pei Dong, Nan Li, Yuwen He, Tiangang Xu, Jianzhong Yin, Jinglin Wang
Published 2017-10-30
Cite as Chinese J Exp Clin Virol, 2017, 31(5): 405-408. DOI: 10.3760/cma.j.issn.1003-9279.2017.05.006
Abstract
ObjectiveTo discuss the taxonomy and molecular characteristics of one virus strain (SZC30) isolated from Culicoides in Yunnan.
MethodsCulicoides were collected with light trap method in the Wulong Village of Shizong County of Yunnan Province in July, 2013. BHK-21 and C6/36 cells were used for virus isolation. The positive isolates were inoculated into brain of one-day suckling mice. Alphavirus and Getah virus specific primers were used to amplify the genome of the virus isolation by RT-PCR. The products of RT-PCR were sequenced. Clustal X1.83, DNAStar, Mega5.1 were used for bioinformatics analysis.
ResultsTotally 3 500 culicoides were collected and divided into 41 batches for virus isolation. One isolate (SZC30) produced cytopathic effect (CPE) on BHK-21 and C6/36 cells; the result of RT-PCR with Alphavirus and Getah virus NS1 specific primer were positive; the sequence analysis of NS1 gene suggested that SZC30 and two Getah virus strains (YN0540, SC1210) from China were in the same evolutionary branching, the nucleotide homology were 97%-100%, and the amino acid was 97%-100%.
ConclusionsSZC30 isolated in Yunnan province was identified as Getah virus.
Key words:
Getah virus; Culicoides; Isolation; Identification
Contributor Information
Pei Dong
School of Public Health, Kunming Medical University, Kunming 650500, China
Nan Li
Yunnan Key Laboratory of Tropical and Subtropical Sciences of Yunnan Animal Science and Veterinary Institute, Kunming 650224, China
Yuwen He
Yunnan Key Laboratory of Tropical and Subtropical Sciences of Yunnan Animal Science and Veterinary Institute, Kunming 650224, China
Tiangang Xu
China Animal Health and Epidemiology Center, Qingdao 266032, China
Jianzhong Yin
School of Public Health, Kunming Medical University, Kunming 650500, China
Jinglin Wang
Yunnan Key Laboratory of Tropical and Subtropical Sciences of Yunnan Animal Science and Veterinary Institute, Kunming 650224, China
