Original Article
Genomic sequence analysis of a hantavirus found in Yunnan bat
Hongyan Deng, Jinglin Wang, Lili Li, Yunyun Xin, Mengmeng Liu, Yun Wang, Zhaojun Duan
Published 2018-04-30
Cite as Chinese J Exp Clin Virol, 2018, 32(2): 140-144. DOI: 10.3760/cma.j.issn.1003-9279.2018.02.007
Abstract
ObjectiveTo understand the novel bat-borne hantavirus on Yunnan province.
MethodsEighty-four bats were collected from Puer, Yunnan from July to August in 2016. A hantavirus, named as DodeHV, was identified in Pomona Roundleaf Bat by high-throughput sequencing technology. Nested or Semi-nested reverse transcription-polymerase chain reaction was used to confirm and screen DodeHV, as well as to amplify its genome sequence by specific primers. Then homology and phylogenetic analysis of all three segments were conducted by using software MegAlign and MEGA 6.0.
ResultsAll the positive individuals are Pomona Roundleaf bats by PCR in this study and 4 out of 67 (5.97%) Pomona Roundleaf bats were positive. The complete ORF of DodeHV S Segment, the most sequence of M and the complete sequence of L segment were obtained. It shared the highest homology with the strain XSV-VN1982B4 found on Phu Tho Province, Viet- Nam in 2013, the nucleotide sequence identities of S, M, L segment compared with XSV was 79.0%, 79.2%, 79.9% respectively, and its amino acid sequence identities was 93.4%, 94.8%, 96.6% respectively. Meanwhile, phylogenetic analysis showed that DodeHV was also closely related to strain XSV-VN1982B4.
ConclusionsThe discovery of DodeHV enriched the virus reservoir of our country which is meaningful to public health. It is indicated that there is potential risk of bat-borne DodeHV at cross-border infection at frontier.
Key words:
Bats; Hantavirus; Phylogenetic analysis
Contributor Information
Hongyan Deng
Department of Pathogeny Biology, Qingdao University Medical College, Qingdao, 266021 China
Jinglin Wang
Yunnan Animal Science and Veterinary Institute/Yunnan Tropical and Subtropical Animal Virus Disease Key Laboratory, Kunming, 650224
Lili Li
National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052
Yunyun Xin
Children Medical Center, The First Affiliated Hospital of Hunan Normal University, Changsha, 410003 China
Mengmeng Liu
Department of Pathogeny Biology, Qingdao University Medical College, Qingdao, 266021 China
Yun Wang
Department of Pathogeny Biology, Qingdao University Medical College, Qingdao, 266021 China
Zhaojun Duan
National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052
