Original Article
Etiological diagnosis and detection of the rabies virus neutralizing antibody in an event of one dog injured seven persons
Liang Cai, Pengcheng Yu, Sihai Li, Fangling He, Hao Yang, Xiaoyan Tao, Guangbin Li, Jiahui Liu, Hong Zhang, Shixiong Hu, Zhifei Zhan, Lihua Wang, Lidong Gao
Published 2018-06-30
Cite as Chinese J Exp Clin Virol, 2018, 32(3): 242-246. DOI: 10.3760/cma.j.issn.1003-9279.2018.03.005
Abstract
ObjectiveTo make etiological diagnosis and evaluate the protective effects of post-exposure prophylaxis(PEP) in an event of one dog injured seven persons.
MethodsDirect immunofluorescence assay (DFA) and nested polymerase chain reaction (PCR) were employed to detect nucleoprotein and nucleoprotein(N) gene of rabies virus in the brain tissues of the dog, the positive samples were sequenced for the full length of N gene of rabies virus, then the homology of the N gene of rabies virus was analyzed after the phylogenetic tree was constructed. Rapid fluorescent focus inhibition test (RFFIT) was applied to detect the rabies virus neutralizing antibodies(RVNA) on day 0, 14 and 40 after PEP.
ResultsThe cerebral, cerebellar and hippocampal tissues were positive by DFA and nested PCR. The phylogenetic tree indicated the rabies virus belonged to the rabies virus genotype I. The homology of the nucleotide and amino acid of the rabies virus N gene were over 86% with the vaccine strains. The titer of the RVNA increased significantly from the day 0 to day 14 after PEP, the lowest was 5.78 IU/ml and the highest was 26.15 IU/ml. On the day 40, the highest RVNA titer was 51.96 IU/ml. No rabies cases occurred in a one year follow-up visit.
ConclusionsNormative PEP can effectively prevent the occurrence of rabies cases.
Key words:
Rabies; Rabies virus; Post-exposure prophylaxis; Etiology; Neutralizing antibody
Contributor Information
Liang Cai
Hunan Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China
Pengcheng Yu
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Sihai Li
Hunan Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China
Fangling He
Hunan Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China
Hao Yang
Hunan Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China
Xiaoyan Tao
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Guangbin Li
Shaoyang Center for Disease Control and Prevention, Shaoyang 422000, China
Jiahui Liu
Hunan Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China
Hong Zhang
Hunan Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China
Shixiong Hu
Hunan Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China
Zhifei Zhan
Hunan Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China
Lihua Wang
State Key Laboratory for Molecular Virology and Genetic Engineering, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Lidong Gao
Hunan Center for Disease Control and Prevention, Key Laboratory of Microbial Molecular Biology of Hunan Province, Changsha 410005, China
