Original Article
Antigenicity and genetic characterization of HA gene in influenza B/Victoria-lineage virus in Beijing 2017-2018
Xiaomin Peng, Shujuan Cui, Jiashen Zhao, Yang Pan, Daitao Zhang, Yimeng Liu, Guilan Lu, Weixian Shi, Quanyi Wang, Peng Yang
Published 2019-08-30
Cite as Chinese J Exp Clin Virol, 2019, 33(4): 337-341. DOI: 10.3760/cma.j.issn.1003-9279.2019.04.001
Abstract
ObjectiveTo understand the antigenicity and genetic characterization of influenza B virus HA gene in B/Victoria-lineage virus (BV) in Beijing during 2017-2018.
MethodsThirty BV virus strains isolated from MDCK cell culture by 17 laboratories in Beijing were collected. The antigenicity was analyzed by comparing with the vaccine strain recommended by WHO. The total viral nucleic acid was extracted and HA gene was amplified by RT-PCR and sequenced. The phylogenetic tree was constructed by HA and mutant sites were analyzed.
ResultsAmong 30 strains of BV, 23 strains (76.7%) were low-reactive strains, other 7 strains (23.3%) were related to the vaccine. The phylogenetic analysis showed that the HA gene of all 30 strains located in Clade 1A branch. In addition, amino acid mutations occurred in 8 sites, and 6 of them located in the antigen determining region.
ConclusionsThere was a correlation between the high proportion of low-reactive antigenicity and 6 aa variation in antigenic determinants involved in HA region of BV influenza virus between 2017-2018, which provides an important laboratory basis for the recommendation of BV influenza vaccine.
Key words:
Influenza virus; Antigenicity; Genetic characterization; Vaccine recommendation
Contributor Information
Xiaomin Peng
Institute for Infections Disease and Endemic Disease Control, Beijing Municipal Center for Disease Control and Prevention, Beijing Research Center for Preventive Medicince, Beijing 100013, China
Shujuan Cui
Jiashen Zhao
Yang Pan
Daitao Zhang
Yimeng Liu
Guilan Lu
Weixian Shi
Quanyi Wang
Peng Yang
