Mechanism of immunogenic cell death induced by microwave ablation in treatment of osteosarcoma
Zhe Yu, Jiachang Wu, Minghua Zhang, Yong Zhou, Qingyu Fan
Published 2016-06-15
Cite as Chin J Radiat Oncol, 2016, 25(6): 602-608. DOI: 10.3760/cma.j.issn.1004-4221.2016.06.013
Abstract
ObjectiveAs a classical approach for hyperthermic ablation, microwave ablation (MWA) has been widely used in the treatment of tumors that cannot be removed by traditional surgery. MWA devitalizes the neighboring tissue and kills tumor cells by thermal diffusion. In the last two decades, this technique has been improved for treating malignant bone tumor in our institute. In situ ablation has already replaced en bloc resection and achieved satisfactory treatment outcomes. This study explores whether tumor cell death induced by MWA would cause the release of immunogenic tumor antigens and tumor-specific immune responses.
MethodsThree models of MWA were established using osteosarcoma cell lines from the mouse, rat, and human, respectively. The expression of immunogenic molecules was measured during in vitro and in situ ablation with different ablation time and group design.
ResultsThe injection of tumor vaccines made from tumor cells or supernatant treated with in vitro ablation resulted in substantial inhibition of tumor cell growth in tumor-bearing animal models. The CD8+ T cells induced by vaccines played a key role in the process. The effector cells released cytokines, IFN-γ and TNF-α, to inhibit tumor cell growth and also trigger Fas/FasL-mediated apoptosis.
ConclusionsMWA-treated osteosarcoma cells can be used to induce specific antitumor immunogenic effects. Therefore, in situ MWA combined with immunotherapy provides an alternative treatment method for patients who have trouble due to their insensitivity to chemotherapy.
Key words:
Immunogenic cell death; Osteosarcoma; Microwave ablation
Contributor Information
Zhe Yu
Department of Orthopedic Surgery, Orthopedics Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China
Jiachang Wu
Department of Orthopedic Surgery, 303rd Hospital of PLA, Nanning 530021, China
Minghua Zhang
Department of Orthopedic Surgery, Orthopedics Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China
Yong Zhou
Department of Orthopedic Surgery, Orthopedics Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China
Qingyu Fan
Department of Orthopedic Surgery, Orthopedics Oncology Institute of PLA, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China