Physics • Biology • Technique
Liver kinase B1 gene enhances radiosensitivity of lung cancer H460 cells: an in vivo study
Hao Li, Wei Zhang, Haoying Huang, Xiangnan Qiu, Shaodong Tong, Xinjun Zhang, Hui Wang, Ruilin Xie, Zhaohui Qin, Yuanhu Yao
Published 2017-09-15
Cite as Chin J Radiat Oncol, 2017, 26(9): 1084-1088. DOI: 10.3760/cma.j.issn.1004-4221.2017.09.021
Abstract
ObjectiveTo investigate the effect of liver kinase B1(LKB1) on the radiosensitivity of subcutaneous xenograft tumor of lung cancer H460 cells in nude mice.
MethodsHuman lung cancer H460 cells were implanted into female nude mice (BALB/c-nu) to establish a subcutaneous xenograft tumor model of lung cancer. A total of 24 female nude mice in which the model was successfully established were equally and randomly divided into four groups: pEGFP-Ctrl plasmid (empty vector plasmid) group, irradiation (IR)+ pEGFP-Ctrl plasmid group, pEGFP-LKB1 plasmid (overexpressing LKB1) group, and IR+ pEGFP-LKB1 plasmid group. The growth of xenograft tumors was observed and the tumor inhibition rate and enhancement factor (EF) were calculated. The expression of LKB1 in each group was measured by immunohistochemistry and Western blot to analyze the relationship between LKB1 and radiosensitivity.
ResultsCompared with the pEGFP-Ctrl plasmid group, the IR+ pEGFP-Ctrl plasmid group, pEGFP-LKB1 plasmid group, and IR+ pEGFP-LKB1 plasmid group showed varying degrees of inhibition of tumor growth, particularly in the IR+ pEGFP-LKB1 plasmid group, and the tumor inhibition rates were 31.30%, 14.78%, and 43.48%, respectively. The EF of LKB1 in the IR+ pEGFP-LKB1 plasmid group was 1.18.The immunohistochemistry and Western blot showed that LKB1 could be effectively expressed in the pEGFP-LKB1 plasmid group and IR+ pEGFP-LKB1 plasmid group, but not in the other two groups.
ConclusionsThe subcutaneous xenograft tumor model of human lung cancer H460 cells has been successfully established in nude mice. LKB1 has a radiosensitizing effect on the subcutaneous xenograft tumor of lung cancer H460 cells in nude mice.
Key words:
Lung cancer cell line; Liver kinase B1; Radiosensitivity; Xenograft
Contributor Information
Hao Li
Department of Radiation Oncology, Affiliated Hosptial of Xuzhou Medical University, Xuzhou, 221002, China
Wei Zhang
Department of Radiation Oncology, Third People’s Hospital of Zhengzhou, Zhengzhou 450000, China
Haoying Huang
Department of Radiation Oncology, Affiliated Hosptial of Xuzhou Medical University, Xuzhou, 221002, China
Xiangnan Qiu
Department of Radiation Oncology, Nanjing General Hospital, Nanjing Military Region, Nanjing 210002, China
Shaodong Tong
Department of Radiation Oncology, Affiliated Hosptial of Xuzhou Medical University, Xuzhou, 221002, China
Xinjun Zhang
Department of Radiation Oncology, Affiliated Hosptial of Xuzhou Medical University, Xuzhou, 221002, China
Hui Wang
Department of Radiation Oncology, Affiliated Hosptial of Xuzhou Medical University, Xuzhou, 221002, China
Ruilin Xie
Department of Radiation Oncology, Affiliated Hosptial of Xuzhou Medical University, Xuzhou, 221002, China
Zhaohui Qin
Xuzhou Medical University School of Public Health, Xuzhou 221004, China
Yuanhu Yao
Department of Radiation Oncology, Affiliated Hosptial of Xuzhou Medical University, Xuzhou, 221002, China
