Objective To detect the fiber structure differences in the corpus callosum and frontal lobe between autism children with high functioning (HFA) and the healthy children. Methods Diffusion tensor image were obtained in 18 HFA patients and 16 normal controls matched with age, sex, intelligent quotient, height and weight. The differences of fiber fractional anisotropy ( FA), fiber apparent diffusion coefficient (ADC), fiber number, average fiber length of the subregions of corpus callosum and the frontal lobe between the two groups were measured by the software of DTIStudio. Results Compared with the control group, the fiber number in the HFA group had a significant decrease in anterior third [ (839 ± 193)vs (630 ±203) ;F =9. 419, P =0. 004]. There was no significant difference in FA value and average fiber length (P ＞ 0. 05). ADC value of the HFA group had a significant increase in anterior third [ (0. 88 ±8.56) x 10-3 mm2/s vs (0. 94 ±6. 98) × 10-3 mm2/s;F =5. 739, P =0. 023], in HFA group's left and right frontal lobes, the FA values were significantly decreased (F = 10. 109, P = 0.003;F = 9. 186,P = 0. 005 ) and the ADC values of long range fibers were significantly increased ( F = 6. 154, P = 0. 019;F = 6. 814, P = 0. 014). Conclusion The results indicate the fibers in anterior third of corpus callosum significantly decrease in autism children with high functioning, which implies low connectivity between the frontal and other lobes.