Relationship between the single nucleotide polymorphisms in has-mir-125a-5p rs12975333 and expression of has-mir-125a-5p in Han Chinese female breast cancer patients
ZHENG Mei-zhu, WEI Ling, ZHENG Gang, MU Dian-bin, YANG Li, ZUO Wen-shu
Published 2011-07-25
Cite as Chin J Gen Surg, 2011,26(07): 596-599. DOI: 10.3760/cma.j.issn.1007-631x.2011.07.021
Abstract
Objective To investigate the relationship between the single nucleotide polymorphisms (SNPs) in has-mir-125a-5p rs12975333 and the expression of has-mir-125a-5p and clinicopathological cheracteristics of female breast cancer in Han Chinese women. Methods Genomic DNA was extracted from peripheral blood lymphocytes. taqman-MGB assay was used to type breast cancer of 338 cases and 338 controls. Expression levels of has-mir-125a-5p in 289 biopsies were examined using stem-loop real-time RTPCR and the clinicopathological cheracteristics of breast cancer were evaluated. Result The gene frequencies (GG,GT,TT) of rsl2975333 in the patients were GG 273 (94. 5% ), GT 16 (5.5%),TT 0(0%), while in breast fibroadenoma and controls there were GG 49 ( 100% ), 338 (100%). The expression level of has-mir-125a-5p in breast cancer(0. 19 ±0. 04) was lower than that in the matched nontumor adjacent tissue specimens (0. 37 ± 0. 05 ) ( P = 0. 04 ) .The expression level of minor T allele of mature miR-125a in breast cancer patients was lower than that in has-mir-125a-5p-GG carying(P =0.022). The expression of has-mir-125a-5p was down-regulated in primary breast cancer, especially in elder patients ( P = 0. 036) and lymph node metastasis groups (P = 0. 001) and with negative ERBB2 (P = 0. 007), ERBB3 (P =0. 04). Conclusions rs12975333 polymorphisms in has-mir-125a-5p gene may work as a risk factor of breast cancer in Han Chinese women. The altered expression of has-mir-125a-5p might play a role in the pathogenesis and progression of breast carcinoma.
Key words:
Breast neoplasms; microRNAs; Polymorphism, single nucleotide
Contributor Information
ZHENG Mei-zhu
Department of Breast Surgery, Shandong Provincial Cancer Research Institute,Jinan 250017,China
WEI Ling
Department of Breast Surgery, Shandong Provincial Cancer Research Institute,Jinan 250017,China
ZHENG Gang
Department of Breast Surgery, Shandong Provincial Cancer Research Institute,Jinan 250017,China
MU Dian-bin
Department of Breast Surgery, Shandong Provincial Cancer Research Institute,Jinan 250017,China
YANG Li
Department of Breast Surgery, Shandong Provincial Cancer Research Institute,Jinan 250017,China
ZUO Wen-shu
Department of Breast Surgery, Shandong Provincial Cancer Research Institute,Jinan 250017,China