Original Article
Experimental study of serum SM22 in the diagnosis of acute intestinal ischemia
Haikun Li, Minhua Wang, Xiaoqing Zhou, Qinghui Yin, Bin Ye, Xiangfu Zeng, Xiaoping Liu, Xiansen Zhu, Xiangtai Zeng
Published 2019-02-25
Cite as Chin J Gen Surg, 2019, 34(2): 151-153. DOI: 10.3760/cma.j.issn.1007-631X.2019.02.015
Abstract
ObjectiveTo evaluate smooth muscle protein of 22 kDa (SM22) in the diagnosis of acute intestinal ischemia.
Methods96 healthy adult SD rats were evenly divided into experimental group and control group, with each group subdivided into 6 subgroups, subject respectively to superior mesenteric artery ligation or sham operation. The venous blood samples were extracted from each group rats′ right heart at 0.5, 1, 2, 4, 8, 12 h after the operation, for SM22 testing and small intestines tissues for direct immunofluorescence staining of SM22.
ResultsThe serum SM22 concentration reached a peak at 4 h(265±15)mg/L, then gradually decreased (P<0.05). The I-FABP was mainly expressed in the epithelium of intestinal mucosa. During the 4 hours of intestinal ischemia, The number of SM22 positive particles did not change. After 4 hours, the number of SM22 positive granules had gradually decreased compared with the control group (all P<0.05).
ConclusionSM22 mainly exists in the smooth muscle of intestinal, during the ischemic necrosis of the intestinal muscle layer SM22 leaks into blood stream, resulting in high serum levels of SM22 facilitating early diagnosis of acute intestinal ischemia.
Key words:
Ischemia; Diagnosis; Smooth muscle protein 22
Contributor Information
Haikun Li
Department of Gastro-intestinal Surgery, The First Hospital of Gan-Nan Medical University, Ganzhou 341000, China
Minhua Wang
Department of Gastro-intestinal Surgery, The First Hospital of Gan-Nan Medical University, Ganzhou 341000, China
Xiaoqing Zhou
Department of General surgery, The Second Hospital of Gan-Nan Medical University, Ganzhou 341000, China
Qinghui Yin
Department of General surgery, The Second Hospital of Gan-Nan Medical University, Ganzhou 341000, China
Bin Ye
Department of General surgery, The Second Hospital of Gan-Nan Medical University, Ganzhou 341000, China
Xiangfu Zeng
Department of Gastro-intestinal Surgery, The First Hospital of Gan-Nan Medical University, Ganzhou 341000, China
Xiaoping Liu
Department of Gastro-intestinal Surgery, The First Hospital of Gan-Nan Medical University, Ganzhou 341000, China
Xiansen Zhu
The Pathology Teaching and Research Section, Gan-Nan Medical University, Ganzhou 341000, China
Xiangtai Zeng
Department of Gastro-intestinal Surgery, The First Hospital of Gan-Nan Medical University, Ganzhou 341000, China
