Original Article
LncRNA C21orF96 promotes the invasion and metastasis of gastric cancer by regulating the expression of miR-875-5p and USF2 genes
Zhou Shicheng, Sun Qinhui, Zhang Hongxia, Lian Guodong, Liu Hongjun, Li leping, Guo Xiaobo
Published 2020-01-25
Cite as Chin J Gen Surg, 2020,35(01): 57-60. DOI: 10.3760/cma.j.issn.1007-631X.2020.01.016
Abstract
ObjectiveTo verify that lncRNA-C21orF96 regulates the expression of miRNA-875-5p and USF2 genes and promotes the invasion and metastasis of gastric cancer.
MethodsRT-PCR was used to measure the expression of lncRNA-C21orF96 related miRNA in gastric cancer cells. pcDNA3. 1 plasmid was used to over-express lncRNA-C21orF96 in KATO-Ⅲ and siRNA was used to knockdown the expression of lncRNA-C21orF96 in SGC-7901, and RT-PCR was used to measure the expression of miRNA-875-5p and USF2 genes; By overexpressing lncRNA-C21orF96 in MKN45, transwell was used to observe changes of cells invasion and migration.
ResultsLncRNA-C21orF96 showed a significant inverse relationship with miR-875-5p, (SGC-7901: 21.19 ±1.09 vs. 3.28 ±0.06, P<0.01; SNU-16: 24.76±2.09 vs. 8.16 ±0.07, P<0.01). In KATO-Ⅲ over-expressing lncRNA-C21orF96, miR-875-5p expression decreased significantly while USF2 expression increased (P<0.01); In SGC-7901 with lncRNA-C21orF96 knockdown, miR-875-5p expression increased significantly while USF2 expression decreased (P<0.05). The number of cells passing through the artificial basement membrane in the experimental group was significantly different from that in the control group (migration: 216.19 ± 2.30 vs. 89.19 ± 4.60, P<0.001; invasion: 146.18 ±5.3 vs. 59.18 ± 2.60, P<0.001).
ConclusionsThe overexpression of lncRNA- C21orF96 significantly reduces the expression of miR-875-5p and promotes the expression of USF2, hence promoting the invasion and metastasis of gastric cancer.
Key words:
Stomach neoplasms; Gene expression regulation; Neoplasm metastasis; Neoplasm invasiveness
Contributor Information
Zhou Shicheng
Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Shandong University, Ji′nan 250021, China
Sun Qinhui
Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Shandong University, Ji′nan 250021, China
Zhang Hongxia
Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Shandong University, Ji′nan 250021, China
Lian Guodong
Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Shandong University, Ji′nan 250021, China
Liu Hongjun
Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Shandong University, Ji′nan 250021, China
Li leping
Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Shandong University, Ji′nan 250021, China
Guo Xiaobo
Department of Gastrointestinal Surgery, Shandong Provincial Hospital, Shandong University, Ji′nan 250021, China
