The tumorigenic property of side population cells in a human gallbladder carcinoma cell line GBC-SD
ZHANG Zhi-fa, QIN Ren-yi, WANG Min, ZHU Feng, SHI Cheng-jian, HE Zhen
Published 2011-09-28
Cite as Chin J Hepatobiliary Surg, 2011,17(09): 775-778. DOI: 10.3760/cma.j.issn.1007-8118.2011.09.025
Abstract
Objective To explore the tumor igenic property of side population cells (SP) from human gallbladder carcinoma cell line GBC-SD. Methods SP and non-SP cells were isolated from GBC-SD staining with Hoechst33342 dye by fluorescence-activated cell sorting (FACS). The soft agar clonal assay and xenograft assay were performed to characterize tumorigenic property of side population cells in vitro and in vivo, respectively. The percentage of SP cells was analyzed by FACS in 5 hu man gallbladder carcinoma specimens. Results The percentage of SP cells accounted for approximately 0.87 % of GBC-SD cells. The clone-formed rates of SP was more frequent than that of non-SP cells (14.74% ± 3.53% vs 5.17% ± 1.05%), there was statistically significant difference (t =2.75,P<0. 05). SP cells could generate tumors with as few as 5 × 103 cells (four of seven animals), whereas at least 1 × 105 non-SP cells were needed to form a tumor (one of seven animals). Re-analysis of SPderived tumors by FACS showed that SP cells under in vivo conditions also have the capacity to regenerate the SP and non-SP fractions. Besides, analysis of Hoechst33342 revealed s small fraction of SP cells, ranging from 0. 27% to 2.3% in gallbladder carcinoma specimens. Conclusion SP cells from GBC-SD are highly tumorigenic similar as the cancer stem cells.
Key words:
Carcinoma of gallbladder; Stem cells; Tumorigenic potential
Contributor Information
ZHANG Zhi-fa
Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China
QIN Ren-yi
Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China
WANG Min
Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China
ZHU Feng
Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China
SHI Cheng-jian
Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China
HE Zhen
Department of Biliary and Pancreatic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P. R. China