Experimental Study
Effect of insulin-like growth factor-I receptor on radiosensitivity of HepG2 cells
Cao Dayong, Yan Zhen, Sun Bingyi, Lin Guoying, Zhang Ning
Published 2019-11-28
Cite as Chin J Hepatobiliary Surg, 2019,25(11): 855-859. DOI: 10.3760/cma.j.issn.1007-8118.2019.11.014
Abstract
ObjectiveTo explore the effect of insulin-like growth factor-Ⅰ (IGF-Ⅰ) receptor on radiosensitivity of HepG2 cells and the underlying mechanism.
MethodsHepG2 cells were divided into the following groups: negative control group, siRNA group, irradiation group and combined group. HepG2 cells were transfected with IGF-Ⅰ receptor siRNA combined with irradiation therapy to investigate the effect on cell proliferation by methyl thiazolyl tetrazolium and cell cycle using flow cytometry. Expression of IGF-Ⅰ receptor, proliferating cell nuclear antigen (PCNA), cyclin-dependent kinases 1(CDK1) and Survivin were detected using Western blotting and Q-PCR.
ResultsThe expression of IGF-Ⅰ receptor in HepG2 cells was decreased significantly after siRNA transfection compared with the control group. After the combinational therapy, cell viability was decreased significantly according with control group [(1.02±0.08) vs. (1.08± 0.10) vs. (0.60±0.07)]; In addition, cell cycle was arrested in G2/M[(20.3±0.3)% vs. (22.6±0.4)% vs. (34.7±0.5)%] and CDK1 expression was reduced significantly. The relative expression of Survivin in siRNA group was lower than negative control group, the difference was statistically significant (P<0.05).
ConclusionInhibition of IGF-Ⅰ receptor can enhance the radiosensitivity of HepG2 cells through cell cycle arrest.
Key words:
Carcinoma, hepatocellular; Radiation tolerance; Cell cycle; Insulin-like growth factor-Ⅰ receptor; Survivin
Contributor Information
Cao Dayong
Department of General Surgery Ⅱ, First Hospital of Qiqihaer, Qiqihaer 161000, Heilongjiang Province, China
Yan Zhen
Department of General Surgery Ⅱ, First Hospital of Qiqihaer, Qiqihaer 161000, Heilongjiang Province, China
Sun Bingyi
Department of General Surgery Ⅱ, First Hospital of Qiqihaer, Qiqihaer 161000, Heilongjiang Province, China
Lin Guoying
Department of General Surgery Ⅱ, First Hospital of Qiqihaer, Qiqihaer 161000, Heilongjiang Province, China
Zhang Ning
Department of Hepatobiliary Surgery, the First Medical Center, Chinese PLA General Hospital, Beijing 100853, China
