The establishment of immunochromatographic tests trips with colloidal gold for early and rapid diagnostic of hemorrhagic fever with renal syndrome
XIONG Li-juan, SU Feng-ju, ZENG Ling-lan, HU Li-hua
Published 2008-06-11
Cite as Chin J Lab Med, 2008,31(06): 699-701. DOI: 10.3321/j.issn:1009-9158.2008.06.023
Abstract
Objective To establish an early,simple and rapid colloidal gold strip method for the detection of IgM against hantavirus nucleocapsid protein in patients with hemorrhagic fever with renal syndrome(HFRS).Methods Purified recombinant Hantavirus nucleoprotein(rNP)was labeled by colloidal gold particles and then sprayed and fixed on fiberglass membrane as the combination pad.Anti-Human IgM(μ-chain specific)antibody produced in goat was fixed in the detection area,and mouse antihantavirus antibody was fixed in the quality control area.Both of them were on nitrocellous membrane strip in tandem.Together with a specimen pad ahead.The conbination pad and the nitrocellous membrane were assembled into a test strip.The colloidal gold strip assay was compared with ELISA for evaluation of specificity and sensitivity.Results The colloidal gold strip tests showed positive in the serum samples from 50 cases of HFRS which was clinically diagnosed and then verified by ELISA within 10-15 minutes.Whereas 30 serum samples of healthy donors have tested negative.Conclusions Our new colloidal gold immunochromatographic test strip method was well concordant with the ELISA assay,but the former was more raoid and simole.It could be used primary medical services.
Key words:
Hemorrhagic fever with renal syndrome; Gold colloid; Reagent strips; Immunoglobulin M; Nucleocapsid proteins
Contributor Information
XIONG Li-juan
Infectious Disease Department,Union Hospital,Tongji Medical College,Huazhong university of Science and technology,Wuhan 430022,China
SU Feng-ju
Infectious Disease Department,Union Hospital,Tongji Medical College,Huazhong university of Science and technology,Wuhan 430022,China
ZENG Ling-lan
Infectious Disease Department,Union Hospital,Tongji Medical College,Huazhong university of Science and technology,Wuhan 430022,China
HU Li-hua
Infectious Disease Department,Union Hospital,Tongji Medical College,Huazhong university of Science and technology,Wuhan 430022,China