Study on the relationship of the expression of the B7-H1 and disease progression in HIV/AIDS patients
SUN Guo-quan, ZHANG Zi-ning, LIU Jing, JIANG Yong-jun, WANG Ya-nan, HAN Xiao-xu, SHANG Hong
Published 2011-07-11
Cite as Chin J Lab Med, 2011,34(07): 623-627. DOI: 10.3760/cma.j.issn.1009-9158.2011.07.011
Abstract
Objective To investigate the level of B7-H1 and its counter-receptor PD-1 expression in mDC and different subsets of T lymphocytes in HIV infected individuals in China and to analyze the correlation between the level of B7-H1/PD-1 and disease progression, and to demonstrate that PD-1/PD-L1-dependent inhibition is operating in HIV infected patients.Methods Percentage of B7-H1 and PD-1 expression in mDC, CD+4 T cells and CD+8 T cells from thirty-six untreated HIV infected patients and 20 health controls were selected and detected by flow-cytometry, its correlations with CD+4 T cell absolute counts and plasma viral loads were analyzed.Results The percentage of B7-H1 expression in mDC, CD+4 T cells and CD+8 T cells (mean 15.21, mean 20.63, mean 13.5)were higher than that of health controls (all P<0.05).The percentage of PD-1 expression in CD+4 T cells and CD+8 T cells (mean 17.91, mean 19.21)were higher than that of health controls (P<0.05, P<0.05). The level of B7-H1 and PD-1 expression were inversely correlated with CD+4 T-cell counts(mDC+B7-H1+:r=-0.647, P<0.01;CD+4B7-H1+:r=-0.489, P=0.002;CD+8B7-H1+:r=-0.372, P=0.026;CD+4PD-1+:r=-0.374, P=0.025;CD+8PD-1+:r=-0.455, P=0.005) and positively correlated with HIV viral load(mDC+B7-H1+:r=0.662, P<0.01;CD+4B7-H1+: r=0.426, P=0.01;CD+8B7-H1+:r=0.531, P=0.001;CD+4PD-1+:r=0.362, P=0.03;CD+8PD-1+:r=0.380, P=0.022).Conclusion The level of B7-H1 and PD-1 expression was associated with HIV disease progression, which provides a useful marker to define disease progression of HIV infection.
Key words:
HIV infections; Acquired immunodeficiency syndrome; Antigens, CD
Contributor Information
SUN Guo-quan
Key Laboratory of Immunology of AIDS, Ministry of Health, Department of Laboratory,First Hospital of China Medical University, Shengyang 110001, China
ZHANG Zi-ning
Key Laboratory of Immunology of AIDS, Ministry of Health, Department of Laboratory,First Hospital of China Medical University, Shengyang 110001, China
LIU Jing
Key Laboratory of Immunology of AIDS, Ministry of Health, Department of Laboratory,First Hospital of China Medical University, Shengyang 110001, China
JIANG Yong-jun
Key Laboratory of Immunology of AIDS, Ministry of Health, Department of Laboratory,First Hospital of China Medical University, Shengyang 110001, China
WANG Ya-nan
Key Laboratory of Immunology of AIDS, Ministry of Health, Department of Laboratory,First Hospital of China Medical University, Shengyang 110001, China
HAN Xiao-xu
Key Laboratory of Immunology of AIDS, Ministry of Health, Department of Laboratory,First Hospital of China Medical University, Shengyang 110001, China
SHANG Hong
Key Laboratory of Immunology of AIDS, Ministry of Health, Department of Laboratory,First Hospital of China Medical University, Shengyang 110001, China