Alternations of CD+4 CD+25 Foxp3 + regulatory T cells in early HIV infected patients in China
ZHANG Zi-ning, HU Si-wen, XU Jun-jie, LIU Jing, JIANG Yong-jun, WANG Ya-nan, SHANG Hong
Published 2011-08-11
Cite as Chin J Lab Med, 2011,34(08): 712-716. DOI: 10.3760/cma.j.issn.1009-9158.2011.08.009
Abstract
Objective To study the alternations of regulatory T cells in early HIV infected patients and its association with disease progression.Methods Fifty-one untreated HIV infected patients were enrolled and divided into 3 groups according to their infection time and CD+4 T cell levels(30 early HIV infected patients,15 typical progressors,6 AIDS patients).Twenty normal controls were enrolled.There were no significant differences between the age and sex among four groups.Blood was drawn by venipuncture from each subject in EDTA tubes and the levels of CD+4 CD+25 Foxp3 + regulatory T cells were detected by FACSAria flow-cytometry.Spearman correlation was used to detect association between CD+4 CD+25 Foxp3 + regulatory T cells and the absolute CD+4 T cells,viral load and activation of T cells.Results The levels of CD+4 CD+25Foxp3+ regulatory T cells showed the tendency of increasing tendency from normal control to early HIV infected patients,asymptomatic HIV infected patients and AIDS patients.Early HIV infected patients was significantly lower than that in AIDS group [3.79(2.11 - 5.43) % vs 8.09(4.90 - 8.90) %,Z = - 2.29,P = 0.022].The levels of CD+4 CD+25 Foxp3 + Treg cells were associated with viral set point(r = 0.479,P =0.038) and inversely associated with CD+4 T cells(r = -0.455,P =0.011) and closely associated with HLA-DR expression on CD+3 T cells(r = 0.533,P = 0.002).Conclusions The ratio of CD+4 CD+25 Foxp3 +regulatory T cells of early HIV infected patients was significantly increased and associated with viral set point and CD+4 T cell counts,which indicate that alternation of regulatory T cell may be an important factor contributing to the disease progression in early HIV infection.
Key words:
HIV; Early HIV infection; Regulatory T cell; Activation
Contributor Information
ZHANG Zi-ning
Key Laboratory of AIDS Immunology of Ministry of Health,Department of Laboratory Medicine,The First Hospital of China Medical University,Shenyang 110001,China
HU Si-wen
Key Laboratory of AIDS Immunology of Ministry of Health,Department of Laboratory Medicine,The First Hospital of China Medical University,Shenyang 110001,China
XU Jun-jie
Key Laboratory of AIDS Immunology of Ministry of Health,Department of Laboratory Medicine,The First Hospital of China Medical University,Shenyang 110001,China
LIU Jing
Key Laboratory of AIDS Immunology of Ministry of Health,Department of Laboratory Medicine,The First Hospital of China Medical University,Shenyang 110001,China
JIANG Yong-jun
Key Laboratory of AIDS Immunology of Ministry of Health,Department of Laboratory Medicine,The First Hospital of China Medical University,Shenyang 110001,China
WANG Ya-nan
Key Laboratory of AIDS Immunology of Ministry of Health,Department of Laboratory Medicine,The First Hospital of China Medical University,Shenyang 110001,China
SHANG Hong
Key Laboratory of AIDS Immunology of Ministry of Health,Department of Laboratory Medicine,The First Hospital of China Medical University,Shenyang 110001,China