To investigate the association of C-reactive protein (CRP) gene polymorphisms and plasma hs-CRP level, and effect on the genetic susceptibility of ischemic stroke (IS).

A case-control study was conducted and 548 patients with acute ischemic stroke and 993 age-matched controls from community-based population were included in this study.Epidemiological questionnaires were managed to collect for demographic information.Blood pressure was measured and blood glucose, triacylglycerol, cholesterol, and high sensitivity C-reaction protein (hs-CRP) were detected.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for genotyping of CRP gene in all participants.

The levels of plasma hs-CRP and the proportion of elevated plasma hs-CRP (≥3.0 mg/L) in the ischemic stroke patients (3.534±3.484) mg/L (43.1%) were significantly higher than those of controls (1.957±2.344) mg/L (16.6%), t=9.475, P<0.01, χ²=128.326, P<0.01.The results of association analysis indicated that rs3093059 and rs3091244 of CPR gene presented statistical associations with ischemic stroke.After correction for confounding factors, ORs (95%CI) of additive model and dominant model of rs3093059 were 0.697 (0.528-0.921), 0.671 (0.487-0.923) respectively.ORs (95%CI) of dominant model of rs3091244 was 0.728 (0.536-0.988).Further analysis indicated the polymorphism of rs876537, rs3093059, rs3091244 of CPR genotyping were significantly associated with plasma hs-CRP elevation (≥3.0 mg/L) both in ischemic stroke patients and in controls (P<0.05).

The CRP genetic polymorphisms were negatively associated with ischemic stroke, and positively corrleted with plasma hs-CRP elevation.However, plasma hs-CRP was positively correlated with ischemic stroke.These results suggested that the plasma hs-CRP levels might be accompanied by ischemic stroke.（Chin J Lab Med，2012,35：916-920）

Stroke; Brain ischemia; C-Rearctive protein; Poly mor phism,single nucleotide