Original Article
Circulating microsome proteins in the pathogenesis of preeclampsia
Yuncong Zhang, Jing Yang, Shuo Yang, Chanjuan Cui, Cheng Zhao, Han Guo, Yangyu Zhao, Jie Zhang, Yuan Wei, Rui Qiao
Published 2019-04-11
Cite as Chin J Lab Med, 2019, 42(4): 255-261. DOI: 10.3760/cma.j.issn.1009-8158.2019.04.006
Abstract
ObjectiveIn this study, we aimed to detect the level of total circulating microparticles (MPs) in pregnant women with preeclampsia (PE) and analyze the proteome of MPs to explore their roles in the pathogenesis and progression of PE.
Methods98 pregnant women with PE, 54 healthy pregnant women, and 51 healthy non-pregnant women were enrolled from December 2016 to June 2018, whose MP levels were detected by flow cytometry and compared. Proteins extracted from the MPs were analyzed by high performance liquid chromatography mass spectrometry.
ResultsThe total MP level of the healthy pregnant group was significantly higher than thatof the non-pregnant group [159.87 (113.25, 218.18)/μl vs 94.10 (53.35, 140.23)/μl, P=0.004], but was not significantly different from that of the PE group. By proteomic profiling, 30 differential proteins were obtained between healthy pregnant women and healthy non-pregnant women, which were closely related to biological processes such as complements, coagulation cascades, angiogenesis and so on; 14 differential proteins were found between PE patients and healthy pregnant women, which were closely related to biological processes such as coagulation cascades, complements and inflammatory reactions, angiogenesis and so forth.
ConclusionsThe level of circulating MPs may reflect the hypercoagulability of preeclampsia. In addition, circulating MPs may be involved in the pathogenesis of PE through various pathways by carrying different proteins, which indicates their potential value in the intervention of PE.
Key words:
Pre-eclampsia; Cell-derived microparticles; Thrombophilia; Proteomics
Contributor Information
Yuncong Zhang
Department of Clinical Laboratory, Peking University International Hospital, Beijing 102206, China
Jing Yang
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
Shuo Yang
Department of Clinical Laboratory, Peking University Third Hospital, Beijing 100191, China
Chanjuan Cui
Department of Clinical Laboratory, Cancer Hospital Chinese Academy of Medical Science, Beijing 100021, China
Cheng Zhao
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
Han Guo
Department of Clinical Laboratory, Peking University Third Hospital, Beijing 100191, China
Yangyu Zhao
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
Jie Zhang
Department of Clinical Laboratory, Peking University Third Hospital, Beijing 100191, China
Yuan Wei
Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
Rui Qiao
Department of Clinical Laboratory, Peking University Third Hospital, Beijing 100191, China